中国全科医学Issue(17)：2101-2104,4.DOI:10.3969/j.issn.1007-9572.2015.17.028
胃癌耐药细胞株SGC7901/阿霉素中miR-185的表达及对细胞多药耐药性的影响
Expression of miR-185 in Gastric Cancer Cell Line SGC7901/ADR and Its Influence on the Drug Resistance of the Cells
摘要
Abstract
Objective To test the expression of miR-185 in gastric cancer tissues and cell lines and to investigate the mechanism of miR-185 on the MDR of gastric cancer. Methods Samples of gastric cancer and para -cancer tissues were obtained from 20 patients who accepted gastric cancer resection operation in the Fourth Hospital of Hebei Medical University from March to May in 2014. The expressions of miR-185 in gastric cancer tissues,para-cancer tissues,SGC7901,ADR resistant cell line SGC7901/ADR,gastric epithelial cell line GES -1 were tested with fluorescent quantitation PCR. Then SGC7901/ADR was transfected by miR -185 mimics or irrelevant control sequence, and sensitivity of transfected SGC7901/ADR to chemotherapeutic drugs( ADR,5-FU,L-OHP)was measured. The expressions of mRNA and their protein in MDR related genes MDR1/P-gp,MRP-1,GST-π,LRP were determined by PCR and Western blotting. Results Relative expressions of miR-185 was(0. 910 ± 0. 142) in para-cancer tissues,and(0. 243 ± 0. 045) in cancer tissue,which showed significant difference(t=20. 025,P<0. 001). Relative expressions of miR -185 in GES -1,SGC7901,SGC7901/ADR cells were (0. 903 ± 0. 117),(0. 630 ± 0. 101),(0. 191 ± 0. 030) respectively,which also showed significant difference( F=46. 850, P<0. 001). Among these cell lines,relative expressions of miR-185 in SGC7901,SGC7901/ADR were lower than that in GES-1,and expressions of miR -185 in SGC7901/ADR was lower than that in SGC7901(P <0. 05). Inhibition rates of ADR,5-FU and L -OHP to SGC7901/ADR cells obviously varied with different transfecting methods ( P <0. 05 ). The inhibitions rate of ADR,5 -FU and L -OHP to SGC7901/ADR transfected by miR -185 mimic were higher than those to SGC7901/ADR transfected by irrelevant control sequence or without transfection. By different transfecting methods,the mRNA and protein expression levels of MDR1/P - gp, MRP -1 and GST - π in SGC7901/ADR were significantly different( P <0. 05 ). The mRNA and protein expression levels of MDR1/P-gp,MRP-1 and GST-πin SGC7901/ADR transfected by miR-185 mimic were lower than those in SGC7901/ADR transfected by irrelevant control sequence or without transfection. Conclusion miR-185 is down-regulated in gastric cancr cells,and upregulating miR-185 can enhance sensitivity of gastric cancer cells to chemotherapeutic drugs. The mechanism may be that miR -185 could regulate the expression of some MDR genes.关键词
胃肿瘤/抗药性,肿瘤/微RNA-185/基因,MDR/体外研究Key words
Stomach neoplasms/Drug resistance,neoplasm/MicroRNA-185/Genes,MDR/In vitro分类
医药卫生引用本文复制引用
李勇,檀碧波,范立侨,赵群,王冬,刘羽,刘庆伟..胃癌耐药细胞株SGC7901/阿霉素中miR-185的表达及对细胞多药耐药性的影响[J].中国全科医学,2015,(17):2101-2104,4.基金项目
国家自然科学基金资助项目（81072033，81372580）；河北省自然科学基金资助项目（C2010000619）；河北省普通高校强势特色学科资助项目（冀教高［2005］52）；河北省科技支撑项目（14277779D）；河北省卫生和计划生育委员会重大医学科研课题 （）
