中国全科医学Issue(18)：2205-2209,5.DOI:10.3969/j.issn.1007-9572.2015.18.021
排石冲剂对大鼠草酸钙结石生成的干预效果及其机制研究
Effects and Mechanism of Paishi Granule on Renal Calcium Oxalate Stone Formation in Rats
摘要
Abstract
Objective To investigate the effects and possible mechanism of Paishi granule on renal calcium oxalate stone formation in rats,and provide the basis for the clinical therapy for urinary calculi. Methods A total of 48 SPF level healthy male Wistar rats were separated into 4 groups by random number table method,including normal group,model group,Potassium citrate - treated group,and Paishi granule - treated group(n = 12 for each). The rat model of renal calcium oxalate stone was induced by intragastrically administrated 2% ammonium chloride( AC),together with 1% ethylene glycol( EG)for free drink. At the same time,each group was treated with corresponding drug. Individual weight and urine volume were recorded;24 h urine was collected for the evaluations of urinary oxalate( Ox),calcium( Ca2 + ),phosphate( P3 + )and magnesium (Mg2 + ) levels. The serum urea nitrogen( BUN),creatinine( Cr),Ca2 + ,P3 + ,Mg2 + levels were determined in each animal. The paraffin sections of kidney were prepared for observing the calcium oxalate deposit and pathological changes under the microscope. Results The 24 h urine volumes in Potassium citrate - treated group and Paishi granule - treated group were significantly higher than those in normal group and model group( P < 0. 05 ),despite a nonsignificant difference between Potassium citrate - treated group and Paishi granule - treated group(P > 0. 05). The levels of 24 h urinary oxalate,Ca2 + and P3 + showed significant differences between any two groups from the four groups(P < 0. 05). The urine Mg2 + levels in previously mentioned two treated groups were significantly lower than that in normal group,but higher than in model group(P < 0. 05), whereas exhibited no significant difference between the two treated groups. The differences of serum Mg2 + and P3 + between any two groups exhibited as statistical nonsignificance( P > 0. 05). The serum BUN and Ca2 + levels in the two treated groups were significantly higher than those in normal group,but lower than in model group(P < 0. 05),whereas the differences between the two treated groups were statistically nonsignificant(P > 0. 05). The serum Cr level demonstrated significant difference between any two groups(P < 0. 05). The scores of calcium oxalate crystallization in the two treated groups were significantly higher than in normal group,conversely,lower than model group( P < 0. 05). Furthermore,the score in Paishi granule - treated group was lower than in Potassium citrate - treated group(P < 0. 05). Conclusion The Paishi granule can significantly decrease the levels of urinary oxalate and serum Ca2 + which facilitate renal calculus formation,while accelerate renal calculus dissolution, consequently,inhibiting the formation of calcium oxalate crystals in urine and renal tissues,thus,protecting the kidneys.关键词
尿路结石/草酸钙/排石冲剂Key words
Urinary calculi/Calcium oxalate/Paishi granule分类
医药卫生引用本文复制引用
曹秋实,巴元明,罗俊华,王洁钰..排石冲剂对大鼠草酸钙结石生成的干预效果及其机制研究[J].中国全科医学,2015,(18):2205-2209,5.基金项目
湖北省卫计委科研重点项目（鄂卫通［2013］20号JX6A09）；湖北省医学领军人才培养工程专项经费资助项目 （）
