中国人兽共患病学报Issue(7)：663-668,6.DOI:10.3969/cjz.j.issn.1002-2694.2014.07.001
1株 asdA缺陷型减毒鼠伤寒沙门氏菌作为核酸疫苗载体的构建与鉴定
Construction and identification of an asdA mutant attenuated Salmonella ty phimurium
摘要
Abstract
In this study ,a wild type Salmonella typhimurium (S .typhimurium) strain was isolated and identified in Hong Kong (S129) ,then the asdA gene was knocked out and replaced with kanamycin resistant gene in a Salmonella typhi-murium strain S129 using the λ RED-mediated recombination method .The constructed mutant asdAΔS129 was validated by culturing in the presence or absence of 2 ,6-diaminopimelic acid (DAP) growth in vitro and evaluating its virulence in BALB/c mice challenge assay .Therefore ,this study has demonstrated that an asdA mutant Salmonella typhimurium has been success-fully constructed .关键词
减毒鼠伤寒沙门氏菌/核酸疫苗载体/λ-RED同源重组Key words
attenuated Salmonella typhimurium/DNA vaccine vector/λ RED-mediated recombination method分类
生物科学引用本文复制引用
于斌,罗语思,彭晓,袁硕峰,牛憨笨,屈军乐,张科..1株 asdA缺陷型减毒鼠伤寒沙门氏菌作为核酸疫苗载体的构建与鉴定[J].中国人兽共患病学报,2014,(7):663-668,6.基金项目
国家自然科学基金资助项目（No ．31200639） （）
