中国人兽共患病学报Issue(7)：739-742,4.DOI:10.3969/cjz.j.issn.1002-2694.2014.07.015
空肠弯曲菌主要外膜蛋白 OMP18的B 细胞抗原表位分析及功能鉴定
Analysis and identification of B cell epitopes of the OMP18 from Campy lobacter jejuni
摘要
Abstract
In this study ,we aimed to understand the sequence characteristics ,transmembrane structures ,line B cell epitopes present in the OMP18 from Campylobacter jejuni ,and provide candidate antigens for the antibody detection and vac-cine development .NCBI/Blast ,TMHMM Server V2 and DNA Star softwares were used for the OMP18 sequence analysis . Based on the ELISA ,the whole bacterial antibody IgG of Campylobacter jejuni was used for the identification of the predicted line B cell epitopes .The OMP18 gene was found conserved in different Campylobacter jejuni strains .The OMP18 was predic-ted to be located on the outer surface of the bacteria .And three line B cell epitopes were determined to be present in the OMP18 protein .As a conclusion ,the OMP18 protein was confirmed to be an important outer membrane protein ;three line B cell epitopes were identified in the OMP18 ,which could be further used for Campylobacter jejuni antibody detection and vaccine development .关键词
空肠弯曲菌/外膜蛋白OMP18/序列分析/表位鉴定Key words
Campylobacter jejuni/OMP18/sequence analysis/epitopes analysis and identification分类
医药卫生引用本文复制引用
楼宏强,胡野,王岚,单小云,盛秀胜,高素华..空肠弯曲菌主要外膜蛋白 OMP18的B 细胞抗原表位分析及功能鉴定[J].中国人兽共患病学报,2014,(7):739-742,4.基金项目
浙江省公益性技术应用研究计划资助项目（No ．2013C33215），金华市科技研究计划资助项目（No ．2013-3-017）和浙江省医药卫生计划资助项目（No ．2013KYA214）共同资助 （）
