中国人兽共患病学报Issue(10):1075-1078,4.DOI:10.3969/cjz.j.issn.1002-2694.2014.10.019
2009-2011年环洞庭湖区主要活禽批发市场低致病禽流感监测情况
Surveillance of low pathogenicity avian influenza viruses in major live poultry wholesale markets around Dongting Lake Region,China,2009-2011
摘要
Abstract
The distribution and prevalence of low pathogenic avian influenza virus in major live poultry wholesale markets around the Dongting Lake region ,China were investigated in our study to propose prevention and control measures on low pathogenic avian flu in the area of live poultry wholesale market .The samples were injected to SPF chicken embryos by allanto-ic cavity ,and then the allantoic fluid were harvested and used for hemagglutination (HA) .If it was positive by HA ,subtypes of the virus would be determined by hemagglutination inhibition (HI) and RT-PCT .We isolated 627 low pathogenic avian in-fluenza viruses in major live poultry wholesale market around Dongting Lake region systematically in winter and spring during 2009-2011 ,and the total separation rate was 22 .2% .The duck swab separation rate of low pathogenic avian influenza was the highest ,which was 24 .6% ,and the following was chicken swab that reached 21 .5% ,and the goose swab separation rate was 11% .We isolated 6 HA subtypes including H3 ,H4 ,H6 ,H9 ,H10 ,and H11 in every live poultry wholesale market ,and the separation rate of H9 ,H6 and H4 subtypes was relatively high ,which could reach 11% ,6 .3% and 3 .4% ,respectively . Those results indicated that recessive infection of low pathogenic avian influenza virus was serious in live poultry wholesale mar-ket around the Dongting Lake area ,and it was a great threat to the occurrence of avian flu .关键词
低致病性禽流感/活禽批发市场/监测Key words
low pathogenic avian influenza/live poultry wholesale market/surveillance分类
农业科技引用本文复制引用
黄建龙,王昌建,邓国华,谭丹,范仲鑫,何世成,朱春霞,汪洪冰,刘道新..2009-2011年环洞庭湖区主要活禽批发市场低致病禽流感监测情况[J].中国人兽共患病学报,2014,(10):1075-1078,4.基金项目
国家重点实验室国家开放基金(SKLVBP201003)Supported by the National Open Fund of the State Key Labo-ratory ()