中国人兽共患病学报Issue(10)：1079-1081,3.DOI:10.3969/cjz.j.issn.1002-2694.2014.10.020
贵州省8例空肠弯曲菌病例的病原学诊断与分析
Etiologic diagnosis and analysis of 8 cases of human campylobacteriosis in Guizhou Province, China
摘要
Abstract
Eight patients with suspected cases of C .jejuni were etiologically diagnosed and analyzed in this study to pro-vide scientific basis for the confirmation of the cases of human campylobacteriosis in Guizhou Province ,China .Blood or feces of 8 suspected patients were employed to isolate bacteria strains .Conventional and multi-PCR techniques were applied to identify suspicious bacteria strains .The C .jejuni strains were analyzed by using Pulsed Field Gel Electrophoresis (PFGE) .Suspicious strains of C .jejuni were isolated from all the 8 suspected patients of campylobacteriosis and anticipated genes fragment were detected with multi-PCR .With the digestion of restriction enzyme SmaI ,the 8 C .jejuni strains were divided into 7 PFGE pat-terns with 7-10 DNA bands .Cluster analysis showed that the gross similarity of 8 strains of C . jejuni was more than 50% . The similarity of PFGE patterns between strain GZ201004 and GZ201005 from diarrhea patients was as high as 100% ,while the similarity of strain GZ201201 and GZ201007 was 66 .7% .Moreover ,C . jejuni were detected from all the suspected pa-tients of campylobacteriosis .PFGE results indicated that strains GZ201004 and GZ201005 were from the same source ,while all the 8 isolates showed PFGE polymorphism .关键词
空肠弯曲菌/贵州/PCR/PFGEKey words
Campylobacter jejuni/Guizhou/PCR/PFGE分类
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韦小瑜,李世军,游旅,田克诚,唐光鹏,王定明..贵州省8例空肠弯曲菌病例的病原学诊断与分析[J].中国人兽共患病学报,2014,(10):1079-1081,3.基金项目
国家科技重大专项（No ．2012ZX10004-212），贵州省疾病预防控制中心青年科技基金（No ．2011-2-4）联合资助Supported by grants from the National Key Program for Infectious Disease of China （No ．2012ZX10004-212） and the Science Foundation for Youths of Guizhou Provincial Center for Disease Control and Prevention （）
