中国人兽共患病学报Issue(12)：1209-1213,5.DOI:10.3969/cjz.j.issn.1002-2694.2014.12.008
DNA条形码技术鉴定我国部分白蛉蛉种
Species identification of partial sandflies from China with DNA barcoding
摘要
Abstract
ABSTRACT:To explore the feasibility of DNA barcoding in the identification of sandflies ,the mitochondrial cytochrome c oxidase subunit I (COI) genes of 9 dominant species in China from three genera in the Phlebotominae were studied .The in‐traspecies and interspecies genetic distances were calculated using the Kimura‐2‐parameter model ,and the phylogenetic trees were reconstructed by neighbor‐joining (NJ) method .Results showed that all of sandflies species were successfully distinguish‐ed by the phylogenetic trees .The average intraspecific genetic distance (0 .8% ) was much less than the average interspecific ge‐netic distance (11 .2% ) .The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes ,which are potentially well utilized in sandflies species identification .关键词
白蛉/COI/DNA条形码技术Key words
sandflies/COI/DNA barcoding分类
医药卫生引用本文复制引用
周正斌,张仪,吕山,施文琦,金长发,朱淮民..DNA条形码技术鉴定我国部分白蛉蛉种[J].中国人兽共患病学报,2014,(12):1209-1213,5.基金项目
国家十二五重大专项（No ．2012ZX10004219）和国家科技重大专项（No ．2012ZX10004220）联合资助 （）
