中国人兽共患病学报Issue(12)：1231-1233,3.DOI:10.3969/cjz.j.issn.1002-2694.2014.12.013
布氏菌苗皮内与划痕接种的效果评价
Effect comparison on intradermal injection and scratch inoculation of Brucella vaccine
摘要
Abstract
ABSTRACT:The guinea pigs were immunized by the Brucella vaccine through intradermal injections and the skin scratch respectively ,and then the immune effects of the two ways were evaluated .Serum samples were collected one month after the last injection and detected for the total IgG titer by interval ELISA .Cell‐mediated immune was evaluated by late‐onset hyper‐sensitivity .The guinea pigs were challenged with Brucella melitensis M5 ,and then were killed to isolated M5 from spleen of each guinea pig to compare the protective effects of two methods of immunization .The ELISA results showed that both of the two methods of immunization could induce strong humoral immune response ,and DTH response to Br‐PPD antigen were 100%in both methods .No significant difference in the immune protective effect of two methods was detected .Results of humoral im‐munity ,cellular immunity and protective effect showed the same effect by intradermal injections and skin scratches .关键词
布氏疫苗/体液免疫/DTHKey words
Brucella vaccine/humoral immunity/DTH分类
医药卫生引用本文复制引用
陈成,魏东,李恪梅,付丽丽,黄长江,王国治..布氏菌苗皮内与划痕接种的效果评价[J].中国人兽共患病学报,2014,(12):1231-1233,3.基金项目
重大新药创制科技重大专项 （）
