中国人兽共患病学报Issue(3)：216-221,6.DOI:10.3969/cjz.j.issn.1002-2694.2015.03.006
基于 RNA-Seq 的羊种布鲁氏菌新转录本与非编码 RNA 鉴定
Identification of novel transcripts and sRNA of Brucella melitensis by RNA-Seq
摘要
Abstract
To identify novel transcripts and sRNA in genome of B .melitensis by transcriptome sequencing ,total RNA were extracted from B .melitensis culture and rRNA were removed .After the addition of adaptor ,RNA was reversely transcribed into cDNA ,which were then subjected to PCR amplification and sequencing .The generated reads were mapped to genome se‐quence of B .melitensis strain 16M .With the mapping results ,novel transcripts and sRNA were identified by bioinformatics methods .Sequencing results analysis showed that genome sequence was covered with the reads with good quality .A total of 773 genes were extended in their 5′and/or 3′ends of their original locations .Sixteen novel transcripts and 241 sRNAs candi‐dates were identified .RT‐PCR showed that some of the sRNAs were differentially expressed under stress conditions .In B . melitensis genome ,there is novel transcript which is not predicted .The sRNA does exist in B .melitensis and were expressed under different conditions .关键词
布鲁氏菌/RNA-Seq/转录组/sRNAKey words
Brucella melitensis/RNA-Seq/transcriptome/sRNA分类
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郭英飞,陈泽良,王玉飞,龚春丽,杨明娟,袁久云,庄妤冰,柯跃华,杜昕颖,汪舟佳..基于 RNA-Seq 的羊种布鲁氏菌新转录本与非编码 RNA 鉴定[J].中国人兽共患病学报,2015,(3):216-221,6.基金项目
国家自然科学基金（81171530），北京市科技新星资助项目（Z131102000413062），北京市自然基金项目（6122030）Supported by the National Natural Science Foundation of China （No ．81171530）， the Beijing Novo Program （No ． Z131102000413062），and the Natural Science Foundation of Beijing （）
