中国人兽共患病学报Issue(3)：240-243,4.DOI:10.3969/cjz.j.issn.1002-2694.2015.03.011
宁波副溶血性弧菌临床株毒力基因与多位点序列分型研究
Virulence gene detection and multi-locus sequence typing of Vibrio parahaemolyticus from patients in Ningbo,China
摘要
Abstract
To investigated the toxin genes distribution and molecular characteristics of Vibrio parahaemolyticus from pa‐tients in Ningbo ,V .parahaemolyticus strains were collected from patients with food poisoning and diarrhea .Thermostable di‐rect hemolysin gene (tdh) and TDH‐related hemolysin gene (trh) were detected by polymerase chain reaction (PCR) .Molecu‐lar characteristics were acquired by multi‐locus sequence typing (MLST ) .Of 248 clinical strains were isolated from 2006 to 2012 .Forty‐eight strains were selected to detect virulence genes and MLST genotyping .Forty‐two isolates were detected as tdh+ and 11 isolates were detected as trh+ .There were 9 STs and one undifferentiated type in Ningbo clinical strains .Thirty‐two strains were classified into ST3 ,5 strains into ST265 and 3 strains into ST120 .ST265 was found in Ningbo strains com‐pared with strains from other regions of China .Strains with tdh+ accounted for the majority in Ningbo clinical strains .Twen‐ty‐five strains of ST3 clone were tdh+ /trh‐.There were 9 STs coexsited in Ningbo clinical strains .ST3 clone was dominant , followed by ST265 and ST120 .Strains with tdh+ /trh‐were dominant in the ST3 clone .The unique ST262 was found in Ning‐bo clinical strains .关键词
副溶血性弧菌/耐热直接溶血素基因/耐热直接溶血素相关溶血素基因/多位点序列分型Key words
Vibrio parahaemolyticus/tdh/trh/multi-locus sequence typing分类
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高红,宋启发,徐景野,郑剑,沈玄艺..宁波副溶血性弧菌临床株毒力基因与多位点序列分型研究[J].中国人兽共患病学报,2015,(3):240-243,4.基金项目
宁波市科学技术局基金项目资助（ No ．2012B82018，No ．2011C50041）Funded by the Ningbo Municipal Science & Technology Bureau （）
