中国人兽共患病学报Issue(3)：255-259,5.DOI:10.3969/cjz.j.issn.1002-2694.2015.03.015
PCR-HRM 方法快速鉴定布鲁氏菌的初步应用
Clinical application of PCR and high resolution melting analysis for rapid identification of Brucella isolates
摘要
Abstract
The aim of this study is to develop a rapid and accurately species typing method for Brucella isolates by using High Resolution Melting (HRM ) analysis .Six pairs of primers were used according to the reference for the sequence of pur‐pose gene .Nineteen biotypes of six species Brucella standard strains were identified by PCR‐HRM analysis and this analysis was used to detect the 35 clinical isolates .Results showed Brucella amplified specific melting curves were different from con‐trasted strains with primer Bspp .The six species Brucella standard strains have own characteristic curve shape from each oth‐ers by PCR‐HRM analysis with five pairs of primers .Thirty‐five clinical isolates of Brucella have entirely consistent with PCR‐HRM curve shape with Brucella melitensis standard strains .So ,PCR‐HRM analysis methods can accurately identify Brucella strains ,especially clinical isolated Brucella melitensis ,and may be used in clinical microbiology laboratories .关键词
布鲁氏菌/分子鉴定/高分辨溶解分析(PCR-HRM )Key words
Brucella/molecular diagnosis/PCR-HRM analysis分类
医药卫生引用本文复制引用
梁雪妮,崔步云,毛玲玲,任微,于静波,薛文成,孟冬娅..PCR-HRM 方法快速鉴定布鲁氏菌的初步应用[J].中国人兽共患病学报,2015,(3):255-259,5.基金项目
辽宁省科技攻关计划资助项目（2011225021）和国家科技重大专项课题（2009ZX10004-209）联合资助（梁雪妮和崔步云同等贡献）Supported by the grant from the Key Technologies R&D Pro-gram of Liaoning Province （No ．2011225021），and the Major National Science and Technology Special Project （）
