中国蔬菜Issue(5)：11-18,8.
番茄果实特异性LYC-B干扰载体的构建及在不同颜色果实中的表达特异性验证
Construction of Cherry Tomato Fruit SpecificLYC-B Interference Vector and Validation of Its Specific Expression in Fruits of Discrepant Colors
摘要
Abstract
The experiment will be conducted to research the specific impacts of lycopene β-cyclase interference on carotenoid metabolism,cherry tomato(Solanum lycopersicum L. var. cerasiforme Alef.) fruit-specific lycopene β-cyclase interference vector was constructed,and the validity in different color fruits were verified. Cherry tomato fruit-specific promoter E8 was amplified according to X13437.1 and fruit-specific vector of E8-pBI121 was constructed,which could be expressed in cherry tomato fruits of pink,red,green and purple colors. According to X86452.1,two different lycopene β-cyclase sequences B1 and B2 were amplified,LYC-B1 with 801 bp from 61 bp to 861 bp and LYC-B2 with 302 bp from 480 bp to 781 bp of LYC-B. The interference vector of pBI121-B1B2 with CaMV 35S promotor and the fruit-specific expression interference vectors of E8- pBI121-B1B2 with E8 promotor were constructed. E8-pBI121,pBI121-B1B2 and E8-pBI121-B1B2 were transferred into Agrobacterium tumefaciens GV3101 with the freeze-thaw method,then transformed into living cherry tomato fruits and leaves by injection. The results of GUS staining 7 days after injection showed that E8-pBI121 expressed in pink,red,green and purple cherry tomato fruits;pBI121-B1B2 expressed in leaves,fruits and seeds,while E8- pBI121-B1B2 expressed just in fruits and seeds.关键词
番茄/类胡萝卜素/E8启动子/CaMV 35S启动子/GUS染色Key words
Cherry tomato/Carotenoid/E8 promoter/CaMV 35S promoter/GUS引用本文复制引用
王巧丽,梁燕,张振才,李翠,李云洲,王玲慧..番茄果实特异性LYC-B干扰载体的构建及在不同颜色果实中的表达特异性验证[J].中国蔬菜,2014,(5):11-18,8.基金项目
陕西省科技统筹创新工程计划项目（2011KTCL02-03），西北农林科技大学农业科技推广基金项目 （）
