中国蔬菜Issue(5):26-32,33,8.
芥菜种质资源遗传多样性和亲缘关系的SRAP分析
SRAP Analysis of Mustard Germplasm Genetic Diversity and Genetic Relationship
摘要
Abstract
The paper analyzed the genetic diversity of 111 mustards 〔Brassica juncea(L.)Czern. et Coss.〕materials using SRAP markers,and recorded their morphological characteristics.21 primer combinations with high polymorphism were screened from 300 SRAP primer combinations.106 polymorphic bands among 150 bands were amplified by 21 SRAP primer combinations,and the polymorphic rate was 70.67%.The SRAP clustering results showed that the genetic similarity of 111 mustards ranged from 0.38 to 0.89,and 111 mustards were divided into 5 groups and several subgroups.The agronomic traits including leaf blade traits,and root,stem,leaf,bolting development were investigated and analyzed.The results showed that the genetic similarity of 111 mustards ranged from 0.39 to 1.00,and they could be divided into 4 groups and several subgroups.These two results were nearly the same,indicating that SRAP markers could be applied in studying the genetic diversity and relationships of mustard germplasm resources.关键词
芥菜/农艺性状/SRAP标记/遗传多样性Key words
Mustard/Agronomic trait/SRAP marker/Genetic diversity引用本文复制引用
李宁,司军,任雪松,宋洪元,李成琼..芥菜种质资源遗传多样性和亲缘关系的SRAP分析[J].中国蔬菜,2014,(5):26-32,33,8.基金项目
国家科技支撑计划项目(2012BAD02B01),重庆市农作物良种创新工程资助项目〔CSTC2012GG(80005)〕,星火计划项目(2011GA811001),中央高校基本科研业务费资金资助项目(XDJK2010C068),中央高校基本科研业务费资金资助项目 ()