中国蔬菜Issue(7)：29-32,33,5.
灰葡萄孢菌胞外大分子分泌物的植物毒性及漆酶性质鉴定
Identification of Phytotoxicity and Laccase Properties of Extracellular Macromolecule Substance from Botrytis cinerea
摘要
Abstract
The macromolecular substances larger than 14 kD in weight, secreted byBotryis cinereawere extracted with dialysis method. These substances were directly inoculated into the leaf blades of tomato and <br> chrysanthemum to detect their phytotoxicity. And the laccase property of theseBotryis cinerea secreta was identified using tannic acid and RB light blue as the indicator. The results showed that the extracellular macromolecule substances secreted byB.cinerea could lead to leaf tissue necrosis, suggesting that they were toxic to the plants. The results also showed that 3 days after inoculation ofB.cinerea, there appeared chromogenic reaction on tannic acid and RB light blue plate. And 6 days later, the plate’s color changed almost completely,indicating that the extracellular macromolecule substance had the properties of laccase.关键词
灰葡萄孢菌/胞外大分子分泌物/植物毒性鉴定/漆酶Key words
Botryis cinerea/Extracellular macromolecule substance/Phytotoxic identification/Laccase引用本文复制引用
陈倩,张国斌,施敏,张照利,鄢波,杨红玉..灰葡萄孢菌胞外大分子分泌物的植物毒性及漆酶性质鉴定[J].中国蔬菜,2015,(7):29-32,33,5.基金项目
国家自然科学基金项目（31260062），昆明学院科研项目 （）
