中国蔬菜Issue(7)：33-39,7.
不同施肥方式对红壤蔬菜田氨氧化细菌和氨氧化古菌群落的影响
Effect of Different Fertilization on Ammonia-oxidizing bacteria and Ammonia-oxidizing archaea in Red Soil Vegetable Field
摘要
Abstract
AdoptingamoA gene clone library construction method,this paper studied the diversity of ammonia-oxidizing bacteria(AOB) and ammonia-oxidizing archaea (AOA)and correlations between diversity index and urease activity in red soil vegetable field by different fertilization including decomposed organic fertilizer (DNPK),fresh organic fertilizer(FNPK),Nitrogen,Phosphorus and Potassium chemical fertilizer(NPK), Phosphorus and Potassium chemical fertilizer(PK).The results showed that OTU quantity,Shannon index of AOB library and AOA library represented organic fertilizer (DNPK and FNPK) were higher than that in chemical fertilizer soil (NPK and PK).The dominant AOB groups in FNPK and DNPK treated soil were Nitrosolobus multiformis,which represented 88.5% and 68.5%,respectively.The main AOB groups in chemical fertilizer soil (NPK and PK) wereNitrosospira sp.with proportion of 54.8% and 65.5%,respectively.The main AOA groups in red earths were CandidatusNitrosotaleadevanaterra,which occupied 90.9% in DNPK treated soil, 84.4% in FNPK treated soil,77.8% in NPK treated soil,and 45.2% in PK treated soil.The urease activity and diversity index in soils treated by DNPK and FNPK were higher than that treated by NPK and PK.There was positive correlation between diversity index of AOA community and urease activity,but there was no marked positive correlation between diversity index of AOB community and urease activity.In conclusion,organic fertilizer was more able to improve the diversity of AOB and AOA communities in red soil of vegetable field than chemical fertilizer.AOA community played more important role in the process of ammoxidation in red soil of vegetable field.关键词
氨氧化细菌/氨氧化古菌/红壤/基因克隆文库Key words
Ammonia-oxidizing bacteria/Ammonia-oxidizing archaea/Red soil vegetable field/Gene clone library引用本文复制引用
周志成,罗葵,唐前君,荣湘民,刘强,何飞飞..不同施肥方式对红壤蔬菜田氨氧化细菌和氨氧化古菌群落的影响[J].中国蔬菜,2015,(7):33-39,7.基金项目
国家自然科学基金项目（31201688，31101413），国家科技支撑计划项目（2012BAD151304），中国博士后科学基金项目（2012M511374），湖南省科技厅一般项目（2012RS4036），湖南农业大学“神农学者”专项 （）
