中国水产科学Issue(3)：474-483,10.DOI:10.3724/SP.J.1118.2014.00474
日本沼虾过氧化物还原酶基因的克隆及其表达分析
Molecular cloning and gene expression of peroxiredoxin (Prx) in ori-ental river pawn (Macrobrachium nipponense) in response to envi-ronmental hypoxia and reoxygenation
摘要
Abstract
Peroxiredoxins (Prxs) are a family of ubiquitous proteins that minimize the harmful effects of oxidative stress by catalyzing the reduction of hydrogen peroxide (H2O2) and organic hydroperoxides to less harmful forms. A full-length cDNA corresponding to a 2-Cys Prx gene was isolated from the oriental river pawn Macrobrachium nippo-nense and designated as MnPrx (GenBank accession no. KC866353). The full-length cDNA was 998 bp, containing a 72 bp 5′untranslated region (UTR), a 212 bp 3′UTR with a poly (A) tail, and a 594 bp open reading frame (ORF) en-coding a polypeptide of 198 amino acids with a molecular mass of 22.131 Da. Like other 2-Cys Prxs, the MnPrx protein possesses two conserved cysteine residues that play an essential role in the antioxidant activity of this proteins. The MnPrx protein, as deduced from the cDNA sequence, has a high level (87%) of sequence similarity to the 2-Cys Prxs from Pacific white shrimp Litopenaeus vannamei. Quantitative real-time RT-PCR analysis revealed that the Prx gene was expressed in the ovary, hepatopancreas, muscle, heart, testis, and intestines, with expression being highest in the hepatopancreas. MnPrx mRNA expression was significantly higher in the hepatopancreas and gill of prawns exposed to hypoxia (12 and 24 h) and reoxygenation (6 h) stress than in the control group. This suggests a possible role in alleviat-ing oxidative stress by increasing Prx mRNA expression in response to environmental hypoxia and reoxygenation.关键词
日本沼虾/过氧化物还原酶/基因克隆/低氧胁迫/定量PCRKey words
Macrobrachium nipponense/peroxiredoxin/molecular cloning/hypoxia/quantitative PCR分类
农业科技引用本文复制引用
孙盛明,戈贤平,傅洪拓,朱健,张世勇..日本沼虾过氧化物还原酶基因的克隆及其表达分析[J].中国水产科学,2014,(3):474-483,10.基金项目
国家“十二五”科技支撑计划项目(2012BAD26B04,2012BAD25B07) （2012BAD26B04,2012BAD25B07）
