中国水产科学Issue(3)：511-521,11.DOI:10.3724/SP.J.1118.2014.00511
豆油替代鱼油对中华绒螯蟹幼蟹生长、非特异性免疫和抗病力的影响
Effect of replacing dietary fish oil with soybean oil on growth, non-specific immune response, and resistance to Aeromonas hydrophila challenge in Chinese mitten crab, Eriocheir sinensis
摘要
Abstract
Traditionally, fish oil is the main lipid source in the feed for most species in aquaculture owing to its effective supply of energy and high content of essential fatty acid. The fatty acid especially n-3 HUFA from the fish oil had also been proved to have positive effect on maintaining the immune system. However, the decline of fish oil production from wild fisheries has resulted in a supply shortage and price escalation of fish oil. Therefore, it has been a major challenge to find viable sources of oil to substitute fish oil. Among available vegetable oils, soybean oil has been widely chosen due to its availability, price and abundant content of fatty acid. In this study, we evaluated the effects of diets with different fish oil and soybean oil ratios on grouth, non-specific immune response and disease resistance in Chinese mitten crab (Eriocheir sinensis). Five isonitrogenous and isoenergetic diets were for-mulated in which 0%(control:F1), 25%, 50%, 75%, or 100%(F2-F5) of the fish oil was replaced with soybean oil. Juvenile crabs (mean weight 0.27±0.03 g) were assigned to one of five treatment groups (four replicates per group) and fed one of the diets for 42 d. There was no significant difference in the survival rate among the treatments (P>0.05). The weight gain was significantly higher in the F4 group than in the F1 group (P<0.05). Similarly, the crabs in the F4 group had significantly higher serum ACP (acid phosphatase), AKP (alkaline phosphatase), and serum SOD (superoxide dismutase) activities than the other treatment groups (P<0.05). F4 and F3 group were significantly higher than the other groups (P<0.05). Serum MDA (malondialdehyde) content was significantly higher in the F1 group than in the other groups, but there was no difference between the other groups (P>0.05). Serum PO (phenoloxidase) activity was higher in F4 and lower in F5 than the other groups (P<0.05). There was no difference in serum lysozyme activity among the groups (P>0.05). After the feeding experiment, the crabs were infected with Aeromonas hydrophila for 10 d. The first death was recorded in F5, and the crabs in F5 had significantly higher cumulative mortality than those in F1 and F4 (P<0.05), but there was no significant difference among the other groups (P>0.05). In conclusion, complete substitu-tion of fish oil with soybean oil did not affect growth performance, but did reduce the ability to resist disease. Our re-sults suggest that replacement of 75%fish oil with soybean oil in the diet of Chinese mitten crab can improve growth with little effect on disease resistance.关键词
中华绒螯蟹/鱼油/豆油/替代/生长/非特异性免疫/幼蟹Key words
Eriocheir sinensis/fish oil/soybean oil/replacement/growth/non-specific immune/juvenile分类
农业科技引用本文复制引用
陈彦良,李二超,禹娜,田文静,江星,孙立梅,齐霁,陈立侨..豆油替代鱼油对中华绒螯蟹幼蟹生长、非特异性免疫和抗病力的影响[J].中国水产科学,2014,(3):511-521,11.基金项目
国家自然科学基金项目(31172422) （31172422）
国家973计划项目(2014CB138603) （2014CB138603）
公益性行业(农业)科研专项(201003020,201203065) （农业）
“十二五”国家科技支撑计划项目(2012BAD25B00) （2012BAD25B00）
上海市中华绒螯蟹现代农业产业技术体系建设项目 （）
上海市科委重点项目(10JC1404100) （10JC1404100）
上海高校知识服务平台上海海洋大学水产动物遗传育种中心项目(ZF1206) （ZF1206）
