中国水产科学Issue(3)：574-580,7.DOI:10.3724/SP.J.1118.2014.00574
基于微卫星标记对长江中上游胭脂鱼增殖放流效果的评估
Effect of restocking enkancement of Chinese sucker in the middle and upper reaches of Yangtze River based on microsatellite loci
摘要
Abstract
Chinese sucker, Myxocyprinus asiaticus (Bleeker), is a freshwater fish that is endemic to China, and the only representative of the Catostomidae in Asia. Historically, M. asiaticus were primarily distributed in the Yangtze and Min-jiang (Fujian Province) rivers. Currently, the only remaining wild population is in the Yangtze River, primarily in the middle and upper reaches. The species was listed as a class Ⅱ state protected species by the Chinese government in 1988 and as a vulnerable species (VU) by the International Union for Conservation of Nature in 1998. Currently, hatch-ery-reared juvenile M. asiaticus are released into natural river systems to supplement the wild population in the Yangtze River. However, the effectiveness of these enhancement efforts has not be evaluated to date. To address this, we col-lected 149 breeding parents and 65 recaptured individuals, and evaluated the parentage relationship using 11 polymor-phic microsatellite loci. A total of 140 alleles were obtained and the mean observed and expected heterozygosity was 0.771 and 0.759, respectively. The range of the non-exclusion probability was 0.356-0.890 and the combined non-exclusion probability (first parent) of 11 loci was 99.998 3%. Eleven of the 65 recaptured individuals were off-spring of the breeding parents (4 from 26 samples in 2011, 3 from 16 samples in 2012, 4 from 23 samples in 2013). Our results suggest that the total contribution of the enhancement to the wild population of M. asiaticus was 16.92%in the middle and upper reaches of Yangtze River.关键词
胭脂鱼/微卫星标记/亲子鉴定/增殖放流/效果评估/长江中上游Key words
Myxocyprinus asiaticus (Bleeker)/microsatellite loci/parentage analysis/restocking enhancement/effect evaluation/middle and upper reaches of Yangtze River分类
农业科技引用本文复制引用
成为为,汪登强,危起伟,王成友,杜浩,吴金明,李雷..基于微卫星标记对长江中上游胭脂鱼增殖放流效果的评估[J].中国水产科学,2014,(3):574-580,7.基金项目
农业部公益性行业科研专项(201203086) （201203086）
