中国水产科学Issue(4)：684-692,9.DOI:10.3724/SP.J.1118.2014.00684
刀鲚MSTN基因的克隆及其组织表达
Cloning and tissue expression of the MSTN gene in Coilia nasus
摘要
Abstract
Estuarine tapertail anchovy (Coilia nasus, junior synonym C. ectenes) are widely distributed throughout the Yangtze River, the coastal waters of China and Korea, and the Ariake Sound of Japan. The species is commercially im-portant because of its nutritional value and taste. In recent years, overharvest and changes in the aquatic ecosystem al-most resulted in the extinction of the species in the middle reaches of the Yangtze River. Researchers have evaluated a number of measures to conserve the species, including captive breeding in ponds, artificial propagation, and larval rearing. As a result, the immediate threat to C.nasus has been alleviated at present. However, artificially cultured C. nasus grow relatively slowly (~125 g in 2+years) so there is a need to improve the growth rate. To determine the mo-lecular mechanisms controlling growth and meat quality in Coilia nasus, we cloned the myostatin gene in C. nasus by homologous cloning methods. Its full-length cDNA was 2 252 bp long, with a 1 125 bp open reading frame (ORF) that encoded a 374 amino acid protein. The C. nasusu MSTN protein was predicted to contain a signal peptide sequence, conserved cysteine residues, and RXXR proteolytic sites. Gene expression was deduced by qRT-PCR. The MSTN gene of C. nasus was expressed strongly in the muscle and brain, but weakly in the gills, liver, spleen, intestine, kidney, and head kidney. MSTN expression in C. nasus was not limited to skeletal muscle. Our results suggest that the biological actions of MSTN in C. nasus, and possibly in other fishes, may not be limited to skeletal muscle growth repression, but may also influence different cell types and organ systems, particularly brain cells. The MSTN gene has shown signifi-cant potential in mammalian breeding programs, and our results provide for a basis for development of breeding pro-grams in C. nasus.关键词
刀鲚/MSTN基因/克隆/组织表达Key words
Coilia nasus/MSTN gene/expression pattern分类
农业科技引用本文复制引用
杜富宽,聂志娟,徐钢春,徐跑,顾若波..刀鲚MSTN基因的克隆及其组织表达[J].中国水产科学,2014,(4):684-692,9.基金项目
国家科技支撑计划项目(2012BAD26B05) （2012BAD26B05）
中央级公益性科研院所基本科研业务费院级专项(2012A0705) （2012A0705）
江苏省水产三新工程重大专项(DZ2012-1) （DZ2012-1）
