中国水产科学Issue(4)：759-768,10.DOI:10.3724/SP.J.1118.2014.00759
酵母β-葡聚糖对黄河鲤免疫应答及抗嗜水气单胞菌感染的影响
Effects of yeastβ-glucan on the immune response and Aeromonas hy-drophila infection resistance in Cyprinus (Cyprinus) carpio haematop-terus
摘要
Abstract
The present study was conducted to investigate the effect of yeast β-glucan (Angel Yeast Co., Ltd, Yichang, China) either by orally (in feed) and injection or β-glucan and Aeromonas hydrophila emulsion-oil vaccine (β-AH) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in Cypri-nus (Cyprinus) carpio haematopterus. And 1 800 healthy C. (C.) c. haematopterus with body weight of (32.33±0.21) g were selected, and randomly divided into 12 And groups (Ⅰ–Ⅻ) with 3 replicates in each group and 50 fish in each replicate. Five levels (150, 300, 450, 600 and 750 mg/kg) of β-glucan were added in the diets of Ⅱ–Ⅵ groups, and controlⅠand Ⅶ–Ⅻ groups were fed with basal diet (0 mg/kg β-glucan), respectively. On the first day (00:00) before the experiment, three different dosages of β-glucan of 150, 450, 750 mg/kg(body weight) of fish were injected intrap-eritoneally to the C. (C.) carpio haematopterus of Ⅶ–Ⅻ groups. Similar, yeast β-glucan was administrated by injection with inactive vaccine which was prepared in carp-derived A. hydrophila and oil emulsion forⅩ,Ⅺ and Ⅻ groups in three doses (1.50 g/L, 4.50 g/L和7.50 g/L) respectively. On the 7 th, 14 th, 28 th, 35 th, and 42 th days of the experiment, LZM, PI and agglutinating antibody titer were determined. On days 43 and 50 the fish were selected and divided into two subgroups under each treatment group for challenge with 2×LD50 concentration of A. hydrophila (44 th day) and subsequently, RPS was recorded on day14 post challenge. The results showed that LZM and PI rose to their highest levels on day 35, and the highest protection in the challenge study after feeding with the diet containing 450 mg (yeastβ-glucan)ּkg−1(diet), however, there was no significant difference in agglutinating antibody titre of C. (C.) carpio haematopterus. On the contrary, the result was observed depressed those parameters in fish feeding with the diet con-taining 750 mg/kg( yeastβ-glucan). The C. (C.) carpio haematopterus injectedβ-glucan andβ-AH significant enhanced the LZM, PI, agglutinating antibody titre and RPS than control and test fish fed with diets supplementedβ-glucan. The results indicated that those parameters rose to their highest levels on 35 days at a medium dose. Injection of high-doseβ-glucan andβ-AH LZM, PI, agglutinating antibody titre and RPS in 42 d gradually strengthen contrast medium. The challenge by 2×LD50 A. hydrophila result showed that the RPS was significantly increased with increasing dietaryβ-glucan up to 450 mg/kg (85.46±7.71, P<0.05), but there were no significant differences forβ-glucan 450 mg/kg to 600 mg/kg (P>0.05), and decreased thereafter with further increases in dietaryβ-glucan. Compared to the dietary sup-plementation, does injected with β-glucan and β-AH had increased RPS. The best RPS of fish was obtained at 450 mg/kg or 4.50 g/L respectively. It could be concluded that the yeastβ-glucan can be used as immunostimulants and en-hancement of immunity and survival of the C. (C.) carpio haematopterus.关键词
酵母β-葡聚糖/黄河鲤/免疫/嗜水气单胞菌Key words
yeastβ-glucan/Cyprinus (Cyprinus) carpio haematopterus/immune/Aeromonas hydrophila分类
农业科技引用本文复制引用
殷海成,杨东辉,贾峰,黄进,张勇..酵母β-葡聚糖对黄河鲤免疫应答及抗嗜水气单胞菌感染的影响[J].中国水产科学,2014,(4):759-768,10.基金项目
河南省重点科技攻关计划项目“黄河鲤仔稚鱼发酵芽孢杆菌饲料生产工艺及应用技术研究”(132102110026) （132102110026）
