中国水产科学Issue(4)：793-799,7.DOI:10.3724/SP.J.1118.2014.00793
生物絮团对仿刺参幼参生长与酶活性的影响
Effect of bioflocs on enzyme activities and growth performance of juvenile sea cucumber Apostichopus japonicus
摘要
Abstract
We evaluated the effects of bioflocs on digestibility, immunity, growth, and survival of juvenile sea cucumber Apostichopus japonicus during a 30 d feeding trial. Animals were fed one of four diets in which 0, 10%, 15%or 20%of the carbon source was replaced with sucrose. Additionally, we tested the effect of water-exchange frequency (3 or 7 d). The experiment was set up using an orthogonal design and we measured growth performance, survival, enzyme activi-ties, and soluble protein content. The activities of amylase(AMS) in the experimental groups were generally higher than in the control, indicating that the bioflocs stimulate amylase activity in A. japonicus. On day 15, superoxide dismu-tase(SOD, 32.9 U/mg prot)and alkaline phosphatase (AKP, 146.8 U/g prot)activity was higher in the 3 d/15% group than in the other three groups and the control(P<0.05). Similarly, SOD (35.3U/mg prot) and AKP(158.8 U/g prot) activ-ity was higher in the7 d/20%group than in the other three groups and the control group(P<0.05). AMS and SOD activ-ity were higher on day 30 than on day 15 in the 7 d/10%group and the increase in AKP activity was particularly sig-nificant. The growth rate (4.12%/d) and survival (98.9%) were highest in this group, whereas survival was lowest (91.8%) in the 3 d/20%group. Furthermore, the soluble protein content in the body wall of A. japonicus was not differ-ent from the other three groups and the control (P>0.05).The soluble protein content (10.9 mg/g) was higher in the 7 d/15%group than in the control(P<0.05), but was not different from the 7 d/10%group. Thus, our results suggest that areduction inwater exchanging frequency and feeding quantity may be beneficial to growth performance and soluble protein accumulation in A. japonicus. Our results provide a basis for determining the optimal feeding quantity and wa-ter-exchanging frequency for juvenile sea cucumber in biofloc technology systems.关键词
生物絮团/仿刺参/消化酶/免疫酶/生长Key words
bioflocs/Apostichopus japonicas/digestive enzymes/immune enzymes/growth performance分类
农业科技引用本文复制引用
张秀珍,李斌,白艳艳,马元庆,刘爱英,刘义豪,宋向军,王忠全,孙珊..生物絮团对仿刺参幼参生长与酶活性的影响[J].中国水产科学,2014,(4):793-799,7.基金项目
山东省农业重大应用技术创新项目(2011-2013) （2011-2013）
水生动物营养与饲料“泰山学者”岗位经费资助项目(2007-2012) （2007-2012）
