中国水产科学Issue(6)：1098-1107,10.DOI:10.3724/SP.J.1118.2014.01098
三角帆蚌内脏团不同部位插核育珠对珍珠囊形成的影响
Effects of different tissue sites insert-nucleus on pearl-sac formation in visceral mass of Hyriopsis cumingii
摘要
Abstract
In order to probe the feasibility of culturing insert-nucleus pearl in visceral mass of freshwater oyster, the different sites of visceral mass were inserted with nucleus in Hyriopsis cumingii. The morphology, formation and nacre deposition of the pearl sac or insert-nucleus positions were observed after inserting nucleus to confirm inserting-nucleus sites of beneficial pearl formation. The 5 locations in visceral mass were chosen (I:front foot-visceral mass, II:middle foot-visceral mass, III:near reproduction gland, IV:near stomach, V:near kidney), and mussel was inserted nucleus at these locations. The pearl sac of Hyriopsis cumingii was sampled in the days of 20th, 50th, 90th, and 150th (thd) with operating nucleus, respectively. Then, the formation and pearl formation were observed by the methods of HE staining and paraffin section. To group II, low columnar simple-epithelial cells were formed firstly in 20 thd after inserting nuc-leus, which was secondary pearl sac. Postoperative 50 thd, similar pearl sac epithelial cells were formed at inserting nucleus positions of groups I, II and III, while they was not been detected at these positions of group IV or V. The large granular matter was found at epithelial cells front of groups II and III. Postoperative 90 thd, a large number of intercel-lular spaces were observed at pearl sac of groups I and III. Epidermal cells with multi-nucleus were increased in groups I and II, and the numbers of granular matter of were increased in epithelial cells of groups II and III. But, the stratified squamous epithelium cells were formed in operating positions of groups IV and V. Postoperative 150 thd, intercellular spaces were decreased in pearl sec of groups I and III. Amass of microvillus were found at epithelial cell free-end of pearl sac in group II. Meanwhile, cell nucleuses were increasing and granular matter were decreasing in groups II or III compared to 90 thd. As the same time, discontinuous low columnar cells in treatment IV were found, but these did not appear in group V. The Hyriopsis cumingii were breed with inserting nucleus. The nacre was obvious on the in-serted-nucleus surface in groups I, II or III at postoperative 50 thd, 90 thd, and 150 thd. The results indicated the depo-sitional nacre was evenly distributed for groups II and III, but group I did not. The thickness of pearls lay in group II (0.85 mm±0.06 mm) significantly increased than them in groups I and III (P<0.05;0.62 mm±0.07 mm, 0.56 mm± 0.03 mm). Otherwise, there wasn’t nacre deposition on the nucleus surface of groups IV and V. This study confirmed that the formation of the pearl sac epithelial cells existed obviously difference by inserting nucleus in different position of Hy-riopsis cumingii. The complete pearl sac structure could be formed at different sites for front foot-visceral mass, middle foot-visceral mass, near reproduction gland, and then the nacre were deposited. Furthermore, inserting nucleus in mid-dle foot-visceral mass was conducive to pearl growth. This research provided the practical and theoretical foundation for cultivating freshwater pearl in visceral mass.关键词
三角帆蚌/内脏团插核/珍珠囊/珍珠质Key words
Hyriopsis cumingii/insert-nucleus in visceral mass/pearl sac/nacre分类
农业科技引用本文复制引用
李文娟,黄凯,李倩,祁晓翔,尚朝,周子睿,付元帅,施志仪..三角帆蚌内脏团不同部位插核育珠对珍珠囊形成的影响[J].中国水产科学,2014,(6):1098-1107,10.基金项目
国家自然科学基金资助项目(31201991) （31201991）
上海市优青项目(ssc11004) （ssc11004）
教育部博士点基金项目(20123104120003) （20123104120003）
上海市高校知识服务平台项目(ZF1206) （ZF1206）
