中国水产科学Issue(6)：1154-1164,11.DOI:10.3724/SP.J.1118.2014.01154
小肽对星斑川鲽幼鱼消化酶活性、抗氧化能力和生化组成的影响
Effects of small dietary peptides on digestive enzyme activity, anti-oxidative capability, and biochemical composition in tissues of juvenile starry flounder Platichthys stellatus
摘要
Abstract
Starry flounder (Platichthys stellatus) with initial body weight of (15.30±0.03) g were fed experimental diets supplemented with six small peptide levels of 0%(control group), 0.25%, 0.50%, 0.75%, 1.0%and 1.5%. Three repli-cates were used for each group with 30 fish per replicate. The experiment lasted for 56 days. In addition to the amylase activities of the hepatopancreas, digestive enzyme activity in three tissues (stomach, intestine and hepatopancreas) of P. stellatus were significantly different among the groups (P<0.05), which showed a trend of an early increase and later decrease with the small peptide levels increasing. The small peptides significantly reduced the maleic dialdehyde (MDA) content of the hepatopancreas and serum (P<0.05), and increased total antioxidant capacity (T-AOC) of serum in the 1.0% and 1.5% small peptide groups. There was no significant difference in superoxide dismutase (SOD) and gluta-thione reductase (GR) of the hepatopancreas (P>0.05). SOD and glutathione peroxidase (GSH-Px) of serum in the1.0%and 1.5%small peptide groups, and GR of serum in the 0.75%and 1.0%groups were all significantly higher than in the control group (P<0.05). The phenylalanine content in the experimental groups was significantly higher than in the con-trol group (P<0.05), and leucine in the 0.50%–1.5%groups was higher than the control group (P<0.05), while no sig-nificant difference was found in other amino acid profiles (P>0.05).∑PUFA in the 0.75%and 1.0%groups, and DHA and EPA in the 0.50%and 1.0%groups were all significantly higher than the control group (P<0.05), while ARA was not affected by the small peptide supplemental levels (P>0.05). Overall, appropriate small peptide supplementation can effectively enhance digestive enzyme activities and antioxidative capability of P. stellatus and improve the fish quality to some extent.关键词
星斑川鲽/小肽/消化酶活性/抗氧化能力/生化组成Key words
Platichthys stellatus/small peptides/digestive enzyme activities/antioxidative capability/bioche-mical composition分类
农业科技引用本文复制引用
王际英,姜柯君,夏斌,柳旭东,王世信,黄炳山,孙永智,张利民..小肽对星斑川鲽幼鱼消化酶活性、抗氧化能力和生化组成的影响[J].中国水产科学,2014,(6):1154-1164,11.基金项目
国家海洋公益性行业科研专项(201205025) （201205025）
山东省水生动物营养与饲料泰山学者岗位经费资助(HYK201004) （HYK201004）
山东省科技发展计划项目(2010GZX20506) （2010GZX20506）
