中国水产科学Issue(6)：1172-1180,9.DOI:10.3724/SP.J.1118.2014.01172
拟穴青蟹ANT2基因在不同温度和盐度条件下的应激表达
Effects of different temperature and salinity on the expression of ade-nine nucleotide translocase 2 (ANT2) mRNA in the mud crab, Scylla paramamosain
摘要
Abstract
The mud crab Scylla paramamosain, a eurythermal and euryhaline species, is a commercially exploited crab inhabiting the southeastern coast of China. In recent years, mud crab aquaculture has suffered as a result of unsettled weather, which has led to a seasonal temperature and salinity changes in the sea water. This study aims to explore the molecular mechanism of cold tolerance in S. paramamosain by examining the expression of adenine nucleotide trans-locase 2 (ANT2) at different temperatures and salinities. ANT belongs to the mitochondrial carrier superfamily (MCF). It is the most abundant inner membrane protein of the mitochondrion and is responsible for the transport of ADP and ATP in the cytoplasm and mitochondrial matrix through the inner mitochondrial membrane. In this study, the relative expression of ANT2 mRNA was detected using real-time quantitative polymerase chain reaction at different tempera-tures (10℃, 15℃, 20℃, 25℃), and low temperatures (10℃, 15℃) combined with salinity (10, 35) in mud crabs (cara-pace width 3.7–5.8 cm, body weight 80–100 g). Real-time qPCR indicated that, at different temperatures, ANT2 was expressed in the hepatopancreas, muscle and gill. The relative expression of ANT2 mRNA was lowest in the hepatopan-creas, at a medium level in muscle, and highest in the gill, and the results indicated that the expression of ANT2 was tissue-specific. With declining temperature, the expression of ANT2 mRNA decreased generally, but at 15℃in the mus-cle (1–12 h) and gill, the expression of ANT2 was significantly higher than that at 20℃(P<0.05). The expression pattern of ANT2 at different temperatures demonstrated that it might be associated with energy metabolism, and participate in stress responses at a certain range of low temperatures, stimulating the expression of ANT2 in the mud crab. At the same temperature, the expression of ANT2 at a salinity of 10 was significantly higher than at the higher salinity of 35 (P<0.05), which indicated low salinity might be helpful for metabolism in mud crabs, enhancing cold resistance. This research might provide theoretical guidance for crab aquaculture and management to some extent.关键词
拟穴青蟹/ANT2/温度/盐度/应激/实时定量PCRKey words
Scylla paramamosain/ANT2/temperature/salinity/stress/real time-PCR分类
生物科学引用本文复制引用
于坤,叶海辉,黄陈翠,巩杰,黄辉洋..拟穴青蟹ANT2基因在不同温度和盐度条件下的应激表达[J].中国水产科学,2014,(6):1172-1180,9.基金项目
福建省自然科学基金资助项目(2012J01147) （2012J01147）
福建省科技计划重点项目(2008N0040) （2008N0040）
