中国水产科学Issue(6)：1260-1267,8.DOI:10.3724/SP.J.1118.2014.01260
塔玛亚历山大藻毒素及其对中国对虾的急性毒性
The toxicity of Alexandrium tamarense to Fenneropenaeus chinensis
摘要
Abstract
Cultured shrimps are often exposed to different toxic algae during rearing practices that may affect survival and quality of the product. This study investigated the toxic effect of Alexandrium tamarense, a dinoflagellate produc-ing paralytic shellfish poison, on Chinese shrimp, Fenneropenaeus chinensis. A mouse bioassay (MBA) method was used to determine the toxicity of the extract from A. tamarense (mouse units, MU/cell), and the acute toxic effect of A. tamarense on F. chinensis was studied. In addition, histopathology observation was conducted in gill and hepatopan-creas after 96 h exposure to 1.0×104 cells·mL−1 of A. tamarense and intramuscular injection with 20 µL the extract from A. tamarense (6.63×104 cells/mL). Toxicity of A. tamarense was low, which was 3.9×10–5 MU/cell. In acute toxicity experiment, the 96 h LC50 of A. tamarense was 1.0×104 cells/mL. Based on the 96 h LC50, the safe concentrations were obtained, which was 1.0×103 cells/mL. Histopathology observation revealed that A. tamarense and the extract could cause some abnormal histological changes in gill and hepatopancreas, respectively, such as cellular swelling and vacu-olated. Our study revealed that pond productivity of the harvested organisms may be affected by A. tamarense. Accor-dingly, the concentration of A. tamarense in the prawn ponds should be controlled below 1.0×103 cells/mL at least. Gills of F. chinensis might be the sensitive tissues to A. tamarense exposure. The lesions in the gills of F. chinensis that im-pedes the occurrence of gaseous interchanges is the most likely cause of shrimp death after A. tamarense exposure. The PSP produced by A. tamarense could cause the lesions in the hepatopancreas of F. chinensis.关键词
塔玛亚历山大藻/中国对虾/麻痹性贝毒/毒性/组织病理学Key words
Alexandrium tamarense/Fenneropenaeus chinensis/paralytic shellfish poisoning/toxicity/histopathology分类
农业科技引用本文复制引用
梁忠秀,李健,李吉涛,谭志军,任海,刘德月..塔玛亚历山大藻毒素及其对中国对虾的急性毒性[J].中国水产科学,2014,(6):1260-1267,8.基金项目
国家高技术研究发展计划:主要养殖甲壳类良种培育(2012AA10A409) （2012AA10A409）
国家虾产业技术体系专项(CARS-47) （CARS-47）
公益性行业(农业)科研专项(201103034) （农业）
山东省自主创新专项:重要海水养殖生物种质创新及疾病阻断剂开发(2013C X80202) （2013C X80202）
