中国水产科学Issue(6)：1268-1276,9.DOI:10.3724/SP.J.1118.2014.01268
大麻哈鱼胚胎耳石的茜素红标记及其效果评价
Otolith tagging and related discussions with alizarin red S in chum salmon (Oncorhynchus keta) at stage of embryos
摘要
Abstract
To explore otolith deposition with alizarin red S (ARS) immersed with conditions of different concentrations (10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L) and the time gradient (8 h, 16 h, 24 h, 32 h), as well as its marking results’ dynamic changes of different relaying days, artificial breeding chum salmon (Oncorhynchus keta) at the latter period of embryos were researched as immersing objects.This study tried for the protection of chum salmon with the approach of supplement for flag discharges, assessment for the releasing effects and provision for technical reference.The results showed that the sagittal and lapillus otoliths can be marked well, they all had great marked results detected with visible light and green laser light.The visible light marks were gradually weakened with the extension of sampling time, and were almost disappeared at the 80 d. And they were preserved with a long fluorescence intensity under the green laser without any sign of abating. Fitting curves were tried to express the relationship between the marking value and concentration for each series of time points. Combined with security assessments, the suitable group marking conditions for chum salmon otoliths at the stage of embryos were drawed, it was that lying the eggs with ARS immersed at the concentration of 25.9 mg/L to 40 mg/L , the time of 15.6 h to 24 h, and they must satisfy conditions that coordinates under the curve of quadratic function y=0.055x2–3.861x+86.9 (x means time, y means concentration). The new increased number of grain rounds were significantly correlated with cultured days in linear shape, and correlation equations of sagittal and lapillus otoliths were 0.965 and 0.924, which indicated that from embryos to late larvae, chum salmon otoliths deposited daily both in sagittal and lapillus.关键词
大麻哈鱼/胚胎期/耳石/茜素红标记/日轮Key words
Oncorhynchus keta/embryo stage/otoliths/marked with alizarin red/daily growth increments分类
农业科技引用本文复制引用
王臣,刘伟,王继隆,战培荣..大麻哈鱼胚胎耳石的茜素红标记及其效果评价[J].中国水产科学,2014,(6):1268-1276,9.基金项目
农业部财政专项物种资源保护项目(2013) （2013）
