中国水产科学Issue(4)：620-629,10.DOI:10.3724/SP.J.1118.2015.140394
牙鲆家系4个选育性状的测定与分析
Determination and analysis of 4 breeding traits in Japanese flounder (Paralichthys olivaceus) families
摘要
Abstract
Japanese flounder (Paralichthys olivaceus) is an important commercially cultured fish in China, Japan, and Korea. The rapid development of the Japanese flounder aquaculture has led to problems with various diseases that have reduced growth rates and caused huge economic loss. One way to address these problems is to cultivate strains with characteristics such as high growth performance, breeding survival rates, and disease resistance. For this purpose, 38 families and a control group were established in 2009. Four to five months after hatching, 33 of the 38 families and the control group were selected for exposure to Vibrio anguillarum. The surviving individuals were marked with fluores-cent-dye and cultivated in two cement pools. Four hundred days after hatching, lymphocystis disease (LD) broke out in one of the pools containing 16 families and the control group. The average rate of incidence was 62.4%; those families (Families 8, 15, 39, and 104) with disease incidence below 40% were defined as disease-resistant. At the same time, 100 fish selected from each family were marked with fluorescent-dye and cultivated in different cement pools. Roughly 580 d after hatching, breed survival rate (580-BSR) and body weight (580-BW) were determined. For the 38 families, the av-erage 580-BSR was 32.0%. There were seven families (Families 5, 8, 27, 75, 90, 119, and 125) with a 580-BSR above 55%, and they were defined as high breed survival rate families. Notably, in 6 of the 7 families, at least one parent was derived from F0750 (Family 50 established in 2007), suggesting that individuals from F0750 may effectively improve 580-BSR. Chi-square tests indicated that body weight (400-BW) and total length (400-TL) of the resistant group were significantly higher than those of the susceptible group (P<0.01). Pearson correlation demonstrated a strong negative correlation (r<−0.6) between 400-BW, 400-TL, and disease incidence of these families, indicating that growth per-formance positively correlated with LD-resistance. Pearson correlation was also used to determine the relationships between the incidence of LD, 580-BSR, 580-BW and the survival rate after infection with V. anguillarum (VSR). There was a strong negative correlation between the incidence of LD and 580-BW (r=−0.790), while VSR was weakly posi-tively correlated with breeding survival rate (r=0.371). In contrast, no significant correlations were found between the other traits (−0.092≤r≤0.185). In conclusion, 4 families with strong disease-resistance against LD and 7 families with high breed survival rate were screened. The correlations between the four breeding traits indicate that selection can be used to jointly improve the occurrence of these four traits in Japanese flounder.关键词
牙鲆/家系/淋巴囊肿病/鳗弧菌/养殖存活率/体重Key words
Paralichthys olivaceus/family/lymphocystis disease/Vibrio anguillarum/breeding survival rate/body weight分类
农业科技引用本文复制引用
王磊,赵永伟,胡灿灿,陈松林,田永胜,邓寒,李文龙,谢明树,牛余泽,庞仁谊..牙鲆家系4个选育性状的测定与分析[J].中国水产科学,2015,(4):620-629,10.基金项目
国家863计划项目(2012AA10A408) （2012AA10A408）
山东省自主创新专项(2013CXC80202) （2013CXC80202）
山东省泰山学者工程专项 （）
2014年度河南省高校科技创新团队支持计划(14IRTSTHN013) （14IRTSTHN013）
