中国水产科学Issue(4)：654-665,12.DOI:10.3724/SP.J.1118.2015.14326
吉富罗非鱼与奥利亚罗非鱼完全双列杂交后代生长性能与肌肉营养成分的比较
Comparative study of the growth and nutrient composition of muscle tissue of two species of tilapia and their reciprocal hybrids
摘要
Abstract
Using a complete diallel mating system, growth and nutrient composition of muscle tissue, expression of lipid metabolism enzyme genes, and the relationship between crude lipid content and mRNA levels of a lipid metabo-lism enzyme were compared between two species of tilapia, Oreochromis niloticus (the GIFT strain of Nile tilapia) and O. aureus. At the end of an experimental period of 100 days, the specific growth rate of pure-bred progeny of GIFT tilapia was significantly higher than that of the two hybrids and the pure-bred progeny of O. aureus. The feed conver-sion rate, hepatosomatic index, and viscerosomatic index of pure-bred progeny of O. aureus were significantly higher than those of experimental groups. There was no significant difference in the moisture, ash, orcrude protein content. Crude lipid content of pure-bred GIFT tilapia progeny was significantly higher than that of other groups. Total amino acids, essential amino acids, and dispensable amino acids of pure-bred O. aureus progeny were significantly lower than other groups. The rate of essential amino acid formation in four F1 generations was consistent with FAO/WHO stan-dards. Saturated, single unsaturated, polyunsaturated, and n-6 polyunsaturated fatty acids of pure-bred GIFT tilapia progeny were significantly higher than those of the two hybrids and the pure-bred progeny of O. aureus. Correlation analysis showed a negative relationship between muscle crude lipid content and mRNA levels of FAS (R2=0.761 5;P<0.01) and G6PD (R2=0.538 7; P<0.05). In contrast, crude lipid content was positively correlated with mRNA levels of LPL (R2=0.782 5; P<0.01) and HSL (R2 =0.562 4, P<0.05). Overall, the results indicated that the growth and nutri-tional composition of muscle of pure-bred O. aureus progeny were significantly lower than of GIFT tilapia or hybrids. Thus, hybridization may increase growth performance of offspring and improve meat quality, as well as enhancing the effect of selective breeding. Although there were no significant differences in mRNA levels of LPL and G6PD between the two hybrids of GIFT tilapia, higher muscle crude lipid content may amplify mRNA levels of LPL and HSL.关键词
罗非鱼/肌肉营养成分/脂肪酸/氨基酸/脂代谢酶Key words
tilapia/nutritional composition of muscle/amino acids/fatty acids/lipid metabolism enzyme分类
农业科技引用本文复制引用
强俊,杨弘,徐跑,何杰,马昕羽,朱志祥,李瑞伟..吉富罗非鱼与奥利亚罗非鱼完全双列杂交后代生长性能与肌肉营养成分的比较[J].中国水产科学,2015,(4):654-665,12.基金项目
“十二五”国家科技支撑计划项目(2012BAD26B03-1) （2012BAD26B03-1）
现代农业产业技术体系建设专项资金项目(CARS-49) （CARS-49）
