中国水稻科学Issue(3)：223-228,6.DOI:10.3969/j.issn.1001-7216.2014.03.001
水稻同源异型域转录因子 OsHox9的反向遗传学分析
Reverse Genetics Analysis of the Transcription Factor OsHox9,a Member of Homeobox Family,in Rice
摘要
Abstract
The homeobox transcription factors play important roles in the growth and development of the plants, involved in regulation of cell differentiation,the architecture of morphogenesis and response to environmental signals in plants.To study the function of these transcription factors in rice,we constructed RNAi vectors of Os Hox9 and analyzed the function of Os Hox9 by the reverse genetics method.The results showed that the height and tiller number of RNAi transgenic plants were decreased in comparison with wild type plants.Real-time PCR analysis showed that Os Hox9 expression level was downregulated in transgenic plants with phenotype.However,the expression level of Os Hox9 in transgenic plants without phenotype was similar to wild type plants,suggesting that the phenotypes of transgenic plants were caused by RNAi effects.In addition,we also analyzed the expression pattern of Os Hox9 in different organs of rice.The results showed that Os Hox9 was expressed in different organs,especially displayed high expression level in stem apical meristems and young panicles.To investigate the subcellular localization of OsHox9,we constructed the fused vector 35S::OsHox9-GFP to introduce into leaves of tobacco.The transient expression result showed OsHox9 was localized on the cell membrane.关键词
水稻/Os Hox9/RNA 干涉/表达分析Key words
rice/Os Hox9/RNAi/expression analysis分类
生物科学引用本文复制引用
艾丽萍,申奥,高志超,李政龙,孙琼琳,栾维江..水稻同源异型域转录因子 OsHox9的反向遗传学分析[J].中国水稻科学,2014,(3):223-228,6.基金项目
国家自然科学基金资助项目(31171515) （31171515）
天津市应用基础及前沿技术研究计划重点项目(11JCZDJC17900) （11JCZDJC17900）
天津市高校中青年骨干创新人才培养计划资助项目(2013-1~2015-12) （2013-1~2015-12）
