中国水稻科学Issue(3)：235-242,8.DOI:10.3969/j.issn.1001-7216.2014.03.003
杂交籼稻骨干保持系岗46B 稻谷粒形及垩白的 QTL 分析
QTL Analysis on Grain Shape and Chalkiness of an Elite Maintainer Line Gang 46B in Hybrid Rice (Oryza sativa L.)
摘要
Abstract
To identify the genetic loci that control grain length,width,length-width ratio,thickness,100-grain weight and the percentage of grains with chalkiness (GL,GW,LWR,GT,HGW,PGWC),we constructed an F2 population by crossing an elite indica maintainer line Gang 46B(G46B)with K1075 ,an introgression line of G46B derived from multi-backcross with an US rice variety Lemont, with low percentage of chalkiness and narrow grain.All six investigated traits followed a continuous phenotypic distribution.Significantly positive or negative correlations were observed between all six traits,except the GL and GW.A genetic map was constructed with 30 simple sequence repeat (SSR)markers and a total of 14 QTLs for grain shape and chalkiness were detected by composite interval mapping (CIM).Among which,two were for GL (qGL2 and qGL5 ),two for GW (qGW5 and qGW8 ),five for LWR (qLWR2 ,qLWR5 ,qLWR6 ,qLWR8 and qLWR12 ),one for GT (qGT5 ),one for HGW (qHGW5 ),and three for PGWC (qPGWC5 ,qPGWC6 and qPGWC8 ).The G46B alleles contributed to increasing GW,GT,HGW and PGWC, while decreasing GL and LWR at all detected loci.The major QTLs controlling GW,GT,GW,PGWC and LWR were located in the same region RM1 7990-RM18004-RM18068 on chromosome 5 ,which was correspondent to the results identified in extreme phenotype plants using linkage and linkage disequilibrium.关键词
水稻/粒形/垩白/数量性状位点/连锁及连锁不平衡Key words
rice/grain shape/chalkiness/quantitative trait locus/linkage and linkage disequilibrium分类
生物科学引用本文复制引用
高方远,邱玲,陆贤军,任鄄胜,吴贤婷,任光俊,曾礼华..杂交籼稻骨干保持系岗46B 稻谷粒形及垩白的 QTL 分析[J].中国水稻科学,2014,(3):235-242,8.基金项目
四川省青年科技创新研究团队专项计划资助项目(2011JTD0022) （2011JTD0022）
四川省财政创新能力提升工程资助项目(2013GXJS-005) （2013GXJS-005）
国家863计划资助项目(2012AA101102-5) （2012AA101102-5）
