中国水稻科学Issue(5)：447-457,11.DOI:10.3969/j.issn.1001-7216.2014.05.001
水稻粉质胚乳突变体 flo7的理化性质及基因定位
Physicochemical Property Analysis and Gene Mapping of a Floury Endosperm Mutant flo7 in Rice
摘要
Abstract
Starch is major storage substance and accounts for 80% of dry weight in rice endosperm.The rice grain quality is partial determined by component,content and granule structure of starch.In this study,starchy mutant designated flo7 ,selected from the progeny of tissue culture of Nipponbare,displayed floury and opaque endosperm. The grain length of flo7 was longer,but grain width,grain thickness and 1000-grain weight were lower than those of its wild type.Compared with the wild type,the flo7 endosperm accumulated a higher level of glucose,fructose and sucrose,but lower level of dry weight.There was no significant difference in protein content between the wild type and flo7 mutant.The amylose content and gel consistency of flo7 were lower than that of wild type,but the alkali spreading value of flo7 was higher.Scanning electron microscopic examinations showed that the mutant endosperms contained irregular,loosely packed,compound starch granules.Genetic analysis indicated that the mutant phenotype was determined by a single recessive nuclear gene.By means of molecular marker technique,flo7 gene was narrowed down to a 95 .1 kb interval which includes 1 3 open reading frames (ORFs)on the long arm of chromosome 12.The results of RT-PCR indicated that the expression levels of some genes involved in starch synthesis were influenced in flo7 mutant.These results suggested that FLO7 plays an important role in the synthesis and regulation of starch in rice.关键词
粉质胚乳突变体/淀粉/精细定位/理化性质/水稻Key words
floury endosperm mutant/starch/fine mapping/physicochemical property/rice分类
生物科学引用本文复制引用
方鹏飞,李三峰,焦桂爱,谢黎虹,胡培松,魏祥进,唐绍清..水稻粉质胚乳突变体 flo7的理化性质及基因定位[J].中国水稻科学,2014,(5):447-457,11.基金项目
国家自然科学基金资助项目(31301303) （31301303）
浙江省自然科学基金资助项目(LY13C130003) （LY13C130003）
中央级公益性科研院所基本科研业务费专项(2012RG002-1) （2012RG002-1）
