中国水稻科学Issue(5)：458-464,7.DOI:10.3969/j.issn.1001-7216.2014.05.002
水稻叶片形态相关基因 RL3（t）的遗传分析和精细定位
Fine Mapping of Gene RL3 (t),Responsible for Leaf Shape Formation in Rice
摘要
Abstract
Two mutants with rolled leaves,derived from the indica cultivar 93-1 1 via the radiation of 60 Co-γ ray in M2 generation,temporally designated as rl3 (t)-1 and rl3 (t)-2 ,were served as materials for exploring the mechanism underlying the rolling leaf characteristic.Morphological analysis showed that, these two mutants have typically adaxially rolled leaves.In addition,when compared with wild type 93-1 1 ,the plant heights and panicle lengths of rl3 (t)-1 and rl3 (t)-2 significantly decreased,as well as the seed setting percentage.Cytological analysis suggests that the rolled leaf phenotype may be caused by the change of number and size of bulliform cells.Genetic analysis indicated that rolled leaf character is controlled by a recessive nuclear gene.Crossing between the two mutants,the F1 [rl3 (t)-1/rl3 (t)-2 ]plants exhibit rolled leaf,suggesting they are allelic.To map the RL3 (t)gene,an F2 population was generated by crossing the rl3 (t)-1 mutants with Wuyunjing 8 as a mapping population.By using simple sequence repeat (SSR) markers and some new designed sequence tagged site (STS)markers,RL3 (t)was initially mapped in the region between the STS marker M3-45 and the SSR marker RM6676 with the genetic distances of 5 .5 cM and 4.4 cM, respectively,on the long arm of chromosome 3.Furthermore,with the enlarged population and more developed STS markers,RL3 (t)gene was finally delimited to a 46-kb long region governed by the STS markers S3-39 and S3-36.关键词
水稻(Oryza sativa L.)/卷叶/RL3 (t)/基因定位Key words
rice (Oryza sativa L.)/rolled leaf/RL3 (t)/gene mapping分类
生物科学引用本文复制引用
郭旻,李荣德,姚健,朱娟,范祥云,王伟,汤述翥,顾铭洪,严长杰..水稻叶片形态相关基因 RL3（t）的遗传分析和精细定位[J].中国水稻科学,2014,(5):458-464,7.基金项目
国家自然科学基金资助项目(31171158) （31171158）
农业部转基因专项(2011ZX08009-003-005) （2011ZX08009-003-005）
江苏省自然科学基金资助项目(BK2012684) （BK2012684）
江苏省六大人才高峰项目 （）
江苏高校优势学科建设工程资助项目 （）
