中国水稻科学Issue(6)：559-569,11.DOI:10.3969/j.issn.1001-7216.2014.06.001
一个抗病性增强的水稻类病变突变体的蛋白质组学研究
A Proteomic Study on a Disease-Resistance-Enhanced Rice Lesion Mimic Mutant
摘要
Abstract
A lesion-mimic mutant in rice (Oryza sativa L.),chloroplastic-H 2 O 2-induced lesion 1 (chl1 ),has enhanced resistance to rice blast and bacterial blight.To understand the molecular mechanisms underlying this phenotype and its resistance responses,a proteomics-based approach was used to identify differentially-expressed proteins between chl1 and its wild type.Using two-dimensional fluorescence difference gel electrophoresis technology and mass spectrometry, 70 protein spots were successfully identified,of which 46 were up-regulated and 24 were down-regulated in the mutant. These differentially-expressed proteins are involved in diverse biological processes including disease resistance,photo-synthesis,oxidation-reduction reaction,amino acid/protein metabolism,chaperoning and carbohydrate metabolism. The complex regulatory network in which these proteins are involved may play an important role in regulating the pro-grammed cell death and the resistance reaction in chl1 .关键词
水稻/类病变突变体/细胞程序性死亡/抗病性/荧光双向差异凝胶电脉Key words
rice/lesion-mimic mutant/programmed cell death/disease resistance/2D-DIGE分类
生物科学引用本文复制引用
韩雪颖,陈剑平,杨勇,余初浪,张文浩,叶胜海,陈斌,程晨,程晔,严成其..一个抗病性增强的水稻类病变突变体的蛋白质组学研究[J].中国水稻科学,2014,(6):559-569,11.基金项目
国家973计划资助项目(2012CB722504) （2012CB722504）
国家自然科学基金青年项目(31101208,31201482) （31101208,31201482）
浙江省自然科学基金重点项目(Z14C140001) （Z14C140001）
