中国水稻科学Issue(2)：119-125,7.DOI:10.3969/j.issn.1001-7216.2015.02.002
水稻抽穗扬花期耐热 QTL（qHTH5）定位及其遗传效应分析
Mapping a QTL(qHTH5)for Heat Tolerance at the Heading Stage on Rice Chromosome 5 and Its Genetic Effect Analysis
摘要
Abstract
Using an interspecific near-isogenic line derived from Oryza sativa ssp.indica (Shuhui 527)×O .rufipogon Griff.(Yuanjiang common wild rice Hehuatang 3)as materials,a QTL for heat tolerance at the heading stage in the heat-tolerant near-isogenic rice line YJ10-03-01 was analyzed.A total of 360 simple sequence repeat(SSR)markers evenly distributed across 12 chromosomes were used to detect polymorphisms between YJ10-03-01 and indica restorer Shuhui 527, and nine were polymorphic.Single marker analysis revealed that markers on chromosome 5 were associated with heat tolerance at the heading stage using seed setting percentage as index for heat tolerance.By interval mapping using F2 population(1 ,027 plants)from YJ10-03-01 ×Shuhui 527,a QTL for heat tolerance was detected on the short arm of chromosome 5 .The QTL explained 8.6% and 1 9.4% of phenotypic variances in the F2 and F3 generations under heat stress (artificial climate chamber),respectively.Recombinant plants were screened for two flanking markers,RM7320 and RM7444,in F3 generation,and substitution mapping suggested that the QTL was located in an interval of about 304.2 kb between markers RM5 92 and RM1 7921 ,we designated the QTL as qHTH5 (quantitative trait locus for heat tolerance at the heading stage on chromosome 5).关键词
水稻(Oryza sativa L.)/耐热性/QTL/基因定位Key words
rice(Oryza sativa L.)/heat tolerance/QTL/gene mapping分类
生物科学引用本文复制引用
曹志斌,谢红卫,聂元元,毛凌华,李永辉,蔡耀辉..水稻抽穗扬花期耐热 QTL（qHTH5）定位及其遗传效应分析[J].中国水稻科学,2015,(2):119-125,7.基金项目
现代农业产业技术体系建设专项资金资助项目(CARS-01-04) （CARS-01-04）
江西省重大科技项目(20114ABF03105 （）
20143ACF60008) （）
江西省科技支撑计划资助项目(20123NNG60187) （20123NNG60187）
