中国水稻科学Issue(2)：159-166,8.DOI:10.3969/j.issn.1001-7216.2015.02.007
外源磷处理对水稻籽粒植酸含量及相关代谢基因表达的影响
The Response of Phytic Acid and Its Expression Profiles in Rice (Oryza sativa L.)Grain as Induced by Phosphorus Supply
摘要
Abstract
Phytic acid (PA)is the main storage form of phosphorus (P)in cereal grains.However,the relationship between external P supply and grain PA accumulation has not yet been fully understood.Two japonica rice cultivars were used to investigate the effects of phosphorus (P)treatments on grain phytic acid (PA)and its relation to inositol phosphate-related genes expression profiles using a culture of detached panicle of rice (Oryza sativa L.).Results showed that high P level caused a significant increase in grain PA,inorganic P (Pi)and total P contents.However,grain weight and PA content per grain were P concentration-dependent,and decreased under high P treatment (12P).High P supply decreased grain Zn and Fe concentrations,and their bio-availabilities.Moreover,P treatment also influenced all four PA related genes expression (RI NO1 ,ITP5/6 K-6 ,I PK2 and I PK1 ),especially for RI NO1 ,a rate-limiting gene in PA synthesis and highly expressed in rice grains.Specifically,RION1 gene expression was up-regulated at optimum P (3P ) concentration, but was significantly down-regulated under high P treatment (12P ), which corresponded with the variation of grain PA content in rice,suggesting RION1 was a key metabolic gene in PA synthesis with P supply.In contrast,only a slight contribution of I PK2 gene was exhibited in PA synthesis induced by P supply.关键词
植酸/稻穗离体培养/三磷酸肌醇合成酶/水稻Key words
phytic acid/detached rice panicle culture/RI NO1/rice (Oryza sativa L.)分类
生物科学引用本文复制引用
苏达,王复标,雷炳婷,王珏,潘刚,程方民..外源磷处理对水稻籽粒植酸含量及相关代谢基因表达的影响[J].中国水稻科学,2015,(2):159-166,8.基金项目
国家自然科学基金资助项目(31071366和31271655) （31071366和31271655）
