中国水稻科学Issue(2)：200-207,8.DOI:10.3969/j.issn.1001-7216.2015.02.012
印度梨形孢对水稻的促生作用及其机理的初探
Preliminary Study on Mechanisms of Growth Promotion in Rice Colonized by Piriformospora indica
摘要
Abstract
Piriformospora indica is a root endophytic fungus, which is able to establish beneficial symbiotic relationships with plant,and it can promote the growth of plants,enhance crop yield.In order to study the impact of P .indica on growth and development of rice,We analyzed the differences in plant height,biomass,tillering,heading, panicle properties,chlorophyll content,nitrate reductase (NR)activity,root activity and expression of growth-related genes between nonglutinous rice colonized and un-colonized by P .indica .The results showed that the plant height, biomass of shoot,the number of tillers,root activity and NR activity of rice colonized by P .indica were significantly higher than that in un-colonized ones.Rice colonized by P .indica headed 4-6 days earlier than the un-colonized rice. The contents of chlorophyll increased by 1 9.77% and 1 7.89% respectively after P .indica treatment for 3 and 4 weeks. RT-PCR analysis showed that the expression of auxin-regulated genes OsIAA 1 3 and YUCCA were up-regulated in leaves of rice colonized by P .indica ,being 1 .49 and 1 .30 folds as high as that of control plants.The expression of NRR ,which negatively regulates the growth of rice,was 58% as high as that of control plants.The results indicated that the growth promotion of rice conferred by P .indica was relevant to chlorophyll content,NR activity,root activity and expression of growth-related genes.P .indica may promote the growth of rice through improving photosynthetic rate,enhancing mineral nutrition absorption and utilization and inducing auxin secretion.关键词
印度梨形孢/水稻/叶绿素/硝酸还原酶/根系活力/生长素Key words
Piriformospora indica/rice/chlorophyll/nitrate reductase/root activity/auxin分类
农业科技引用本文复制引用
吴金丹,陈乾,刘晓曦,林福呈,高其康,楼兵干..印度梨形孢对水稻的促生作用及其机理的初探[J].中国水稻科学,2015,(2):200-207,8.基金项目
公益性行业农业科研专项(201303022) （201303022）
