中国水稻科学Issue(3)：305-310,6.DOI:10.3969/j.issn.1001-7216.2015.03.010
抗稻瘟病 Pi2/9/z-t 基因特异性分子标记的开发
Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t
摘要
Abstract
Specific molecular markers for detecting the alleles of Pi2/Pi9/Piz-t ,designated as Pi2SNP,Pi9SNP and Pizt-PA,respectively,have been successfully developed through sequence comparison method.These three specific markers can distinctly distinguish target genes from others mapped on Pi2/9 locus,which facilitate the molecular marker-assistant selection and the development of pyramiding cultivars combined with other allele R genes. Additionally,101 main cultivars and breeding parents collected from different rice-planting areas have been diagnosed with these three specific markers,and the results showed that only 2 out of 101 cultivars carried Piz-t ,but neither Pi2 nor Pi9 band had been detected.So far,it indicated that most of the main cultivars and breeding parents in China do not carry Pi2/Pi9/Piz-t .These results had provided important information for the application of Pi2/Pi9/Piz-t in the blast resistance breeding programs.关键词
水稻/稻瘟病/抗性基因/分子标记/分子标记辅助选择育种/Pi2/9位点Key words
rice/blast/resistance gene/molecular marker/MAS/Pi2/9 locus分类
农业科技引用本文复制引用
华丽霞,汪文娟,陈深,汪聪颖,曾烈先,杨健源,朱小源,苏菁..抗稻瘟病 Pi2/9/z-t 基因特异性分子标记的开发[J].中国水稻科学,2015,(3):305-310,6.基金项目
国家自然科学基金资助项目(31301304,31101403) （31301304,31101403）
国家科技(转基因)重大专项(2014ZX0800904B) （转基因）
公益性行业科研专项(201203014) （201203014）
广东省自然科学基金资助项目(S2012010010018) （S2012010010018）
国家重点实验室开放课题资助项目(SKL-CUSAb-2013-10) （SKL-CUSAb-2013-10）
现代农业产业技术体系建设专项(CARS-01-24,粤财教[2009]356号) （CARS-01-24,粤财教[2009]356号）
