中国水稻科学Issue(3)：311-318,8.DOI:10.3969/j.issn.1001-7216.2015.03.011
稻曲病菌 T-DNA 插入突变体 B1464插入位点分析
Molecular Characterization of T-DNA Integration into Ustilaginoidea virens Mutant B1464
摘要
Abstract
With an avirulent Ustilaginoidea virens strain B1464 as material,we analyzed the changes in biological morphology and pathogenic mechanism.Compared to the wild-type U .virens strain P1 ,the mutant strain B1464 showed no pathogenicity in the field.The rate of growth on MM medium was not different to P1 and B1464.On PSA and TB3 medium the rates of growth were nutritionally endowed.B1464 grew slower and produced few conidiophores in PS medium, and its conidiophores morphology and spore size became smaller.Genomic Southern bolt analysis confirmed that B1464 was single T-DNA insertional events.The flanking sequences of T-DNA obtained by TAIL-PCR were non-adjacent in the wild type.It was founds that the mutant strain lost about 20 kb sequences in the insertion site comparing with the wild type strain.The expression of the flanking genes of the T-DNA was down-regulated,detecting by semi-quantitative RT-PCR.The reason of the chromosome rearrangement and abnormal expression of some genes is the insertion of T-DNA,probably,leading to the mutant strain B1464 lost its pathogenicity.关键词
稻曲病菌/T-DNA 插入突变/致病力/苹果酸合成酶/铜转运蛋白Key words
Ustilaginoidea virens/T-DNA insertion mutant/pathogenicity/malate synthase/copper transporter分类
生物科学引用本文复制引用
王亚会,刘永锋,陆凡,俞咪娜,黄磊,郑梦婷,于俊杰,尹小乐..稻曲病菌 T-DNA 插入突变体 B1464插入位点分析[J].中国水稻科学,2015,(3):311-318,8.基金项目
国家自然科学基金资助项目(31171802) （31171802）
江苏省农业自主创新基金资助项目[CX(15)5029] （15）
