中国水稻科学Issue(4)：335-342,8.DOI:10.3969/j.issn.1001-7216.2015.04.001
水稻褐色颖壳新等位基因 OsFBX310 bh6的克隆鉴定
Identification of OsFBX310 bh6,A New Allele for Brown Hull in Rice
摘要
Abstract
A brown hull 6 (bh6 )mutant of rice was isolated from an EMS-induced IR64 mutant bank.The brown pigments start to accumulate in the hulls after heading and reach a maximum level in mature seeds.The 1000-grain weight and panicle length of bh6 significantly decreased while other agronomic traits including plant height,number of productive panicles per plant,number of filled grains per panicle and seed-setting rate were largely similar to those of the wild-type IR64.Analysis of pigment contents indicated that the levels of total flavonoids and anthocyanin in bh6 were significantly increased.The brown hull phenotype was controlled by a single recessive gene on the long arm of chromosome 9.Sequencing analysis indicated that a single base substitution (G-A)at position 101 3 was detected in the candidate gene (LOC_Os09g121 50)encoding an F-box domain containing protein.Functional complementation using the wild-type allele could rescue the mutation.The mutation,designated as OsFBX310 bh6 ,is a new allele of OsFBX310 bh6 provides useful experimental materials and would facilitate the studies on mechanism of flavonoid metabolism in monocot plants.关键词
水稻/褐色颖壳突变体/分子标记/OsFBX310/类黄酮代谢Key words
Oryza sativa L./brown hull mutant/molecular marker/OsFBX310/flavonoid metabolism分类
生物科学引用本文复制引用
徐霞,张晓波,施勇烽,王惠梅,黄奇娜,奉保华,李小红,郭丹,吴建利..水稻褐色颖壳新等位基因 OsFBX310 bh6的克隆鉴定[J].中国水稻科学,2015,(4):335-342,8.基金项目
国家863计划资助项目(2014AA10A603-15,2012AA101102,2011AA10A101) （2014AA10A603-15,2012AA101102,2011AA10A101）
浙江省超级稻重点实验室项目(2013E10021) （2013E10021）
