中国社区医师Issue(17)：8-9,2.DOI:10.3969/j.issn.1007-614x.2014.17.3
HPLC-ELSD法测定血府逐瘀口服液中苦杏仁苷的含量
Content determination of amygdalin in Xue Fu Zhu Yu oral liquid by HPLC-ELSD
孙琦 1仲崇琳 2杨美林2
作者信息
- 1. 133700吉林敖东药业集团股份有限公司 吉林省敦化市
- 2. 130051吉林省中医药科学院 吉林省长春市
- 折叠
摘要
Abstract
Objective:To develop a method for the content determination of amygdalin in Xue Fu Zhu Yu oral liquid.Methods:High performance liquid chromatography-evaporative light-scattering detector method was used.The chromatographic column was octadecylsilane chemically bonded silica as the filler,the moving phase was methanol-water(25:75),the flow velocity was 1ml one minute,the column temperature was 30℃.The drift tube temperature of evaporative light-scattering detector was 105℃,air or nitrogen was as the atomizing gas,its flow velocity was 3.0L one minute.Results:The incoming sample amount of amygdalin was 0.856~4.28 μ g,the natural logarithm of peak area and the natural logarithm of incoming sample amount had good linear relationship(r=0.9995),the average recovery was 99.06% ,RSD=1.39(n=6).Conclusion:This method is sensitive,accurate,can be used for the content determination of amygdalin in Xue Fu Zhu Yu oral liquid.关键词
血府逐瘀口服液/苦杏仁苷/高效液相色谱法/蒸发光散射检测器/含量测定Key words
Xue Fu Zhu Yu oral liquid/Amygdalin/High performance liquid chromatography/Evaporative light-scattering detector/Content determination引用本文复制引用
孙琦,仲崇琳,杨美林..HPLC-ELSD法测定血府逐瘀口服液中苦杏仁苷的含量[J].中国社区医师,2014,(17):8-9,2.
