中国社区医师Issue(22)：7-8,14,3.DOI:10.3969/j.issn.1007-614x.2014.22.2
阿托伐他汀对血管平滑肌细胞中转染的TNF-α启动子活性影响的研究
Study of the effect of atorvastatin on the transfection of TNF-α promoter activity in vascular smooth muscle cell
摘要
Abstract
Objective:To explore the anti-inflammatory action mechanism on transcriptional level of atorvastatin(Atv).Methods:The plasmid DNA had luciferase as the reporter gene and human TNF alpha promoter.The plasmid DNA was transfected to smooth muscle cell in vitro culture.It was stimulated by AngⅡand disposed by different concentrations of Atv.The change of the promoter activity was observed by detecting the activity of Luciferase.Results:The promoter could highly efficient and stable expression in VSMCs.While VSMCs was stimulated by AngⅡ,TNF-α promoter activity was obviously up-regulated than without stimulation(P<0.05).0.1~10 μ mol/L Atv could inhibit the higher expression of TNF-α promoter activity in VSMCs,and it was a dose-dependent manner(P<0.05).Conclusion:The inhibition of Atv on the TNF-α promoter activity proves its potential inhibition of proinflammatory cytokines at transcriptional level.关键词
阿托伐他汀/血管平滑肌细胞/肿瘤坏死因子/炎性反应Key words
Atorvastatin/Vascular smooth muscle cell/Tumor necrosis factor/Inflammatory response引用本文复制引用
程莹,鲁永织,孙敏,张卓琦..阿托伐他汀对血管平滑肌细胞中转染的TNF-α启动子活性影响的研究[J].中国社区医师,2014,(22):7-8,14,3.基金项目
本课题受国家自然科学基金(No.30800219，No.30670557)，江苏省高校自然科学基金(No.08KJD320013)和徐州医学院院长专项人才基金(No.07KJZ26)资助 （No.30800219，No.30670557）
Fundation item This project was supported by the national natural science foundation of China(No.30800219, No.30670557), the college natural science foundation of Jiangsu Province(No.08KJD320013) and dean special talent fund(No.07KJZ26) （No.30800219, No.30670557）
