中国社区医师Issue(31)：92-93,2.DOI:10.3969/j.issn.1007-614x.2014.31.58
胶体金法检测HBsAg漏检原因分析及预防措施
Undetected reasons analysis and prevention measures of colloidal gold method in the detection of HBsAg
摘要
Abstract
Objective:To explore the undetected reasons and prevention measures of colloidal gold method in the detection of hepatitis B surface antigens(HBsAg) in volunteer donors.Methods:The sample with negative colloidal gold method test result was applied enzyme linked immunosorbent assay(ELISA method) for detection.The sample with positive test result was applied again colloidal gold method for detection.The undetected reasons of colloidal gold method were analyzed.Results:In 46 000 cases of volunteer donors,ELISA method detected 226 cases of HBsAg positive,and the positive rate was 0.49%.226 cases of ELISA method positive samples were applied again colloidal gold method for detection,and 58 cases were positive.Conclusion:Methodological limitations and man-made factors are the undetected main reasons of screening HBsAg by colloidal gold method. Strict reagent screening and evaluation before use,improving medical personnel testing skills and quality awareness,strengthening responsibility can reduce the leak detection of colloidal gold method.关键词
HBsAg/胶体金法/酶联免疫吸附试验Key words
HBsAg/Colloidal gold method/Enzyme linked immunosorbent assay引用本文复制引用
朱岷..胶体金法检测HBsAg漏检原因分析及预防措施[J].中国社区医师,2014,(31):92-93,2.
