摘要
Abstract
Objective:Using the rat hepatic ischemia reperfusion injury(HIRI) model,to investigate the ω-3 fatty acid pretreatment on the protective mechanism.Methods:60 rats were randomly divided into the sham operation group(S group),the liver ischemia reperfusion injury model group(HIRI group) and ω-3 polyunsaturated fatty acids(ω-3 PUFAs) pretreatment before ischemia-reperfusion group(ω group),HIRI rat model was established,it was given the ischemia 60 min,samples were prepared after the restoration of blood flow after reperfusion for 1 and 6 hours.In each group,venous blood was drawn at the end of reperfusion,rats were sacrificed to collect the liver tissue.We observed the serum aspartate aminotransferase(AST),alanine aminotransferase(ALT) content;we observed the pathological changes of liver tissue stained by HE;enzyme linked immunosorbent assay was used to measure the rat serum superoxide dismutase(SOD) content;immunohistochemistry method was used to detect the expression level of nuclear transcription factor(NF-κ B) in liver tissue.Results:Compared with S group,in HIRI group and ωgroup,serum ALT,AST level,MDA content and NF-κB expression were increased significantly,while serum SOD activity was significantly decreased(P<0.05),liver tissue pathology damage was obvious;compared with HIRI group,in the ω group,serum ALT,AST activity and NF-κB expression were significantly reduced,and the activity of serum SOD was significantly elevated(P<0.05),liver histopathological injury was lighter.Conclusion: ω-3 PUFAs preconditioning had protective effect on rats HIRI,the mechanism may be to reduce the production of oxygen free radicals and reduce lipid peroxidation,and then make inhibition of NF-κB activation.关键词
肝/HIRI/NF-κB/ω-3 PUFAsKey words
Liver/HIRI/NF-κB/ω-3 PUFAs
