中国社区医师Issue(10)：5-6,2.DOI:10.3969/j.issn.1007-614x.2015.10.1
与胰岛共培养诱导肌源性干细胞分化为胰岛素分泌细胞的可能性研究
The possibility of muscle-derived stem cells to differentiate into insulin-producing cells was induced by co-culture of islet
王欣燕1
作者信息
- 1. 617000四川省攀枝花市第二人民医院内分泌科
- 折叠
摘要
Abstract
Objective:To investigate the possibility of the induction of muscle-derived stem cells(MDSCs)to differentiate into insulin-producing cells by islet and muscle-derived stem cells in neonatal rats(MDSCs)which were co-cultured in vitro.Methods:The primary cells of MDSCs in neonatal rats were extracted and identified,exogenous green fluorescent protein(GFP) were used to transfect MDSCs of the second generation.Rat pancreatic islets were extracted and then identified by dithizone,islets were co-cultured with MDSCs transfected by GFP.While only MDSCs were cultured as the control group.Results: Cell mass was found under a microscope on the fifth days of the co-culture group,and typical pancreatic insulin-secreting cell clusters was formed on the seventh day.Cell clusters identified by dithizone: a part of green cells was dyed scarlet,which indict that scarlet mass coming from MDSCs,but the same changes were not detected in the control group.Conclusion:MDSCs in neonatal rats can be induced into differentiate insulin-producing cells by co-culture MDSCs and islets.关键词
成体干细胞/共培养/胰岛/胰岛素分泌细胞Key words
Muscle-derived stem cells/Co-culture/Pancreatic islets/Insulin-producing cells引用本文复制引用
王欣燕..与胰岛共培养诱导肌源性干细胞分化为胰岛素分泌细胞的可能性研究[J].中国社区医师,2015,(10):5-6,2.
