中国生物防治学报Issue(3)：370-375,6.
哈茨木霉Thga1基因敲除突变株对几种植物病原真菌的离体拮抗作用
Antagonism of the Thga1 Gene Knock-out Mutants of Trichoderma harzianum against Three Kinds of Plant Pathogenic Fungi in vitro
摘要
Abstract
Trichoderma harzianum Th-33 antagonize against a variety of plant pathogenic fungi. Thga1 (GenBank JN874387) of strain Th-33 codes a type I GTP binding protein α subunit. Two Thga1 gene knock-out mutantsΔThgal 1-1 and ΔThgal 10-1 had been constructed in our previous studies. The antagonistic effects of the two mutants against three plant pathogens including Rhizoctonia solani, Phytophthora capsici and Fusarium oxysporum were studied in this paper. The two mutants could not cover and mycoparasitize the colonies of tested pathogens and the antagonisms reduced significantly comparing to wild-type Th-33 when confronted with tested plant pathogens. The inhibitory ratios of volatile metabolites fromΔThgal 1-1 andΔThgal 10-1 to the tested plant pathogens reduced 50%-57%to R. solani, 43%-58%to P. capsici and 58%-63%to F. oxysporum comparing to that of wild-type Th-33 respectively, and there was no significant difference between the two mutants. Inhibition ratios of non-volatile metabolites fromΔThgal 1-1 andΔThgal 10-1 to the three tested plant pathogens reduced 8%-25%to R. solani, 23%-56%to P. capsici and 38%-50%to F. oxysporum, respectively. The parasitism of wild-type Th-33 against R. solani such as hyphae winding, adhering, penetration and leading to hyphae disruption were observed under microscope, but we have not observed the above phenomena resulting from the two mutants. The results showed that Thga1 influenced antagonism, secondary metabolism and mycoparasitism of T. harzianum.关键词
哈茨木霉Th-33/Thga1基因敲除突变株/植物病原菌/拮抗作用Key words
Trichoderma harzianum Th-33/Thga1 gene knock-out mutant/plant pathogen/antagonism分类
农业科技引用本文复制引用
刘增亮,李梅,张玉杰,张林,杨晓燕,蒋细良..哈茨木霉Thga1基因敲除突变株对几种植物病原真菌的离体拮抗作用[J].中国生物防治学报,2014,(3):370-375,6.基金项目
国家自然科学基金项目（31071728）；国家“863”计划项目（2011AA10A205）；公益性行业（农业）科研专项（201303045）；国家“948”计划项目 （）
