中国生物防治学报Issue(3)：385-392,8.
防治青枯病工程菌Hrp-菌株的固体剂型研究
Solid Formulation of Hrp-Mutant of Ralstonia solanacearum as Biocontrol Agent of Bacterial Wilt
摘要
Abstract
Study on solid formulation of Hrp- mutant was explored. The preparation method of Hrp- mutant solid formulation was worked out through screening various carriers, protection agents and antibacterial agents. The preparation process was evaluated in this paper. At first, bacterial cultures after fermentation were collected by centrifugation at 5000 r/min for 10 min, washed twice in sterile water and then suspended in mineral oil, followed by shaking evenly for 15-20 min. After that, the solid formulation was prepared by mixing the suspension with sterilized peat with the proportion of 1:1. Finally, nisin and natamycin were added into the mixture to inhibit other miscellaneous microbes. The prepared solid agent survived for 6 months under the temperature of 25 ℃, pH 5.3, water content of 35%and without vacuum. The survival rate could sustain at 44.4%, and the biomass reached 1.12×1010 cfu/g.关键词
Hrp-菌株/固体剂型/载体/保护剂/抑菌剂Key words
Hrp-mutant/solid formulation/carriers/protection agents/microbe inhibitors分类
农业科技引用本文复制引用
冉淦侨,田云龙,戴佳锟,张丽,郭萍,朱昌雄..防治青枯病工程菌Hrp-菌株的固体剂型研究[J].中国生物防治学报,2014,(3):385-392,8.基金项目
陕西省科学院青年人才培养专项（2012K-31）；国家“948”计划项目 （）
