中国生物防治学报Issue(4)：446-452,7.
外源过氧化氢对菜蛾盘绒茧蜂抗氧化酶和子代滞育的影响
Effect of Exogenous Hydrogen Peroxide on the Antioxidant Enzymes inCotesia vestalis and Diapause of Their Progeny
摘要
Abstract
It has been reported that, with the prolonged photophase in the photoperiod experienced, the content of hydrogen peroxide (H2O2) in the prepupae ofCotesia vestalis(Haliday)increased, while the diapause incidence in their progeny decreased. In order to further explore the function of H2O2 in the diapause induction process ofC. vestalis, we treated the prepupae obtained under a short photoperiod of 8L:16D with different concentration of H2O2 and measured the content of hydrogen peroxide and activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), as well as the diapause incidence in their progeny. The results showed that when the prepupae were treated with exogenous H2O2, the diapause incidence of their progeny declined when they were reared under a short photoperiod at 13 °C. The diapause incidence in the progeny generation decreased from 83.76% in the control group to 35.22% in the group treated with 1.6 mol/L exogenous H2O2, close to the diapause incidence (12.24%) of their progeny whose maternal generation were reared under a long photoperiod. The content of H2O2 in the prepupae increased with the increasing concentration of applied exogenous H2O2, similar to the effect of prolonged rearing photoperiod; while the activities of SOD decreased and those of CAT in the prepupae increased with the increasing concentration of exogenous H2O2, just opposite to the effects with increased rearing photoperiod.The POD activity showed no significant difference between the prepupae treated at a low exogenous H2O2 concentration (0.2 mol/L) and the control. However, when the prepupae were treated at a high concentration (1.6 mol/L), the POD activity was the same as or even lower than the control at 4 h post-treatment, but was much higher at 20 h after treatment. The results indicated that exogenous H2O2 treatment may play a similar role as the long photoperiod in the process of diapause induction inC. vestalis, by preventing diapause occurrence, but has a different way in the effects on the three antioxidant enzymes.关键词
菜蛾盘绒茧蜂/过氧化氢/抗氧化酶/滞育/母代效应Key words
Cotesia vestalis/hydrogen peroxide/antioxidant enzymes/diapause/maternal effects分类
农业科技引用本文复制引用
贺宜鹏,陈超,柴跑,施祖华..外源过氧化氢对菜蛾盘绒茧蜂抗氧化酶和子代滞育的影响[J].中国生物防治学报,2014,(4):446-452,7.基金项目
农业部“948”项目（2011-G4）；国家“973”计划项目（2009CB119005）；国家公益性行业（农业）科研专项 （）
