中国生物防治学报Issue(4)：503-510,8.
棉隆与粉红螺旋聚孢霉67-1协同防治黄瓜枯萎病的研究
Management of Cucumber Fusarium Wilt via Combinative Application of Dazomet and Clonostachys rosea 67-1
摘要
Abstract
We investigated the synergistic effects of soil fumigation with Dazomet and biocontrol fungus Clonostachys rosea67-1 used successively for control of cucumber fusarium wilt in protected fields. Pot experiments showed that death rate of cucumber seedlings in soil infested withFusarium oxysporumf. sp. cucumerinum KW2-1 (FOC) at 3% (w/w) was zero at the 30th day after sowing whenC. rosea67-1 was applied at 106 spores/gsoil following soil fumigation with Dazomet at 1 g/kg of soil, while those were 13.3% and 44.5% in soil treated with Dazomet and 67-1 alone, respectively. Experiments in an infested sunlight greenhouse demonstrated that soil fumigation with Dazomet at 25 g/m2 in combination with hole application ofC. rosea at 107 spores/hole when transplanting led to 98.5% of control efficiency, much higher than those resulted from treatments of fumigation (88.8%) and biocontrol (62.2%). Cucumber yield was increased by 48.5% by combinative treatment. Analysis of Bliss Independence indicated that there was a synergistic effect of Dazomet andC. rosea 67-1 in combating cucumber fusarium wilt. Tests with soil dilution plates showed that population of FOCdeclined drastically and remained at a low level during the whole growing season and the colonization of 67-1 in rhizosphere enhanced greatly (P<0.01) after chemical fumigation followed by application of the 67-1. The research indicated that combination of fumigation and biocontrol could protect cucumber from infection by FOC and might also be used for management of other soil born diseases.关键词
土壤熏蒸/生物防治/协同效应/粉红螺旋聚孢霉/黄瓜枯萎病/土壤微生物区系Key words
fumigation/biological control/synergistic effect/Clonostachys rosea/cucumber Fusarium wilt/soil microflora分类
农业科技引用本文复制引用
田甜,孙漫红,李世东..棉隆与粉红螺旋聚孢霉67-1协同防治黄瓜枯萎病的研究[J].中国生物防治学报,2014,(4):503-510,8.基金项目
国家“863”计划项目（2011AA10A205）；国家科技支撑计划（2012BAD19B01）；行业技术体系项目 （）
