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印楝内生放线菌鉴定及对稻瘟病菌拮抗作用研究

张丽 纪明山 谷祖敏 田宏哲 李修伟 李兴海

中国生物防治学报Issue(4):534-539,6.
中国生物防治学报Issue(4):534-539,6.

印楝内生放线菌鉴定及对稻瘟病菌拮抗作用研究

Identification of Endophytic Actinomycete fromAzadirachta indicaA. Juss. and Its Effect onMagnaporthe grisea

张丽 1纪明山 1谷祖敏 2田宏哲 1李修伟 1李兴海1

作者信息

  • 1. 沈阳农业大学植物保护学院,沈阳110161
  • 2. 辽宁省生物农药工程技术研究中心,沈阳110161
  • 折叠

摘要

Abstract

Ninety-three endophytic actinomycetes were isolated from the leaf and fruit of Azadirachta indica A. Juss.. The results showed that 29 strains had antagonistic activities against Magnaporthe griseaby measuring mycelial growth rate method. The strain YL-2 had a strong antifungal activity againstM. grisea with the inhibition rate of 82.65%. And it exhibited antagonistic activities against 18 kinds of plant pathogenic fungi by cylinder-plate method. The pot experiment showed that the control efficiency of filtrate from YL-2 fermentation liquid onM. griseawas significantly higher than 25 mg/L Kasugamycin WP which could be up to 85.41%. According to the morphological characteristics, physiological and biochemical properties, chemical composition of the cell wall and 16S rDNA, the strain YL-2 was preliminarily identified asStreptomyces rochei.

关键词

印楝/内生放线菌/娄彻氏链霉菌/拮抗作用/稻瘟病菌

Key words

Azadirachta indica/endophytic actinomycete/Streptomyces rochei/antagonism/Magnaporthe grisea

分类

农业科技

引用本文复制引用

张丽,纪明山,谷祖敏,田宏哲,李修伟,李兴海..印楝内生放线菌鉴定及对稻瘟病菌拮抗作用研究[J].中国生物防治学报,2014,(4):534-539,6.

基金项目

国家自然科学基金 ()

中国生物防治学报

OA北大核心CSCDCSTPCD

2095-039X

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