中国生物防治学报Issue(5)：593-599,7.
苹果酸脱氢酶与异柠檬酸脱氢酶在滞育七星瓢虫中的差异表达
Differential Expression of Malate Dehydrogenase and Isocitrate Dehydrogenase in Diapaused Ladybird, Coccinella septempunctata L
摘要
Abstract
To analyze the categories and functions of the diapause related proteins in ladybird, Coccinella septempunctata, proteomics methods, such as two-dimensional electrophoresis, ESI-QUAD-TOF and bioinformatics, were used to identify the proteins with more than 2 folds of expression quantity. Mascot database search determined two matching proteins which showed different expression and are associated with the Krebs cycle, malate dehydrogenase (gi|212508346) and isocitrate dehydrogenase (gi|21392222), which were up-regulated and down-regulated, respectively. We analyzed the possible reasons and provided a theoretical basis for the diapauses environmental regulation at the protein level.关键词
七星瓢虫/滞育相关蛋白/苹果酸脱氢酶/异柠檬酸脱氢酶Key words
Coccinella septempunctata/diapause related proteins/malate dehydrogenase/isocitrate dehydrogenase分类
农业科技引用本文复制引用
刘遥,张礼生,陈红印,黄凤霞,蒋莎,任小云..苹果酸脱氢酶与异柠檬酸脱氢酶在滞育七星瓢虫中的差异表达[J].中国生物防治学报,2014,(5):593-599,7.基金项目
公益性行业（农业）科研专项（201103002）；国家“948”项目（2011-G4）；国家“973”计划 （）
